Detailed information of TA system TA187816

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3906154..3906989
• Replicon: chromosome
• Accession: NZ_CP068799
• Organism: Escherichia coli strain RIVM_C017887

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q83W73
• Locus tag: JNN46_RS18705
• Protein ID: WP_000854700.1
• Coordinates: 3906154..3906531 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JNN46_RS18710
• Protein ID: WP_001531802.1
• Coordinates: 3906621..3906989 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN46_RS18675	3901476..3901970	+	495	WP_001059463.1	MarR family transcriptional regulator	-
JNN46_RS18680	3902405..3902743	-	339	WP_000772639.1	Arm DNA-binding domain-containing protein	-
JNN46_RS18685	3903109..3904428	+	1320	WP_000144686.1	site-specific integrase	-
JNN46_RS18690	3904521..3905375	-	855	WP_024174313.1	DUF4942 domain-containing protein	-
JNN46_RS18695	3905460..3905657	-	198	WP_000839247.1	DUF957 domain-containing protein	-
JNN46_RS18700	3905669..3906157	-	489	WP_000761726.1	hypothetical protein	-
JNN46_RS18705	3906154..3906531	-	378	WP_000854700.1	TA system toxin CbtA family protein	Toxin
JNN46_RS18710	3906621..3906989	-	369	WP_001531802.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN46_RS18715	3907152..3907373	-	222	WP_000692353.1	DUF987 domain-containing protein	-
JNN46_RS18720	3907442..3907918	-	477	WP_001186199.1	RadC family protein	-
JNN46_RS18725	3907934..3908419	-	486	WP_000213723.1	antirestriction protein	-
JNN46_RS18730	3908511..3909329	-	819	WP_001234709.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / vat	3858218..3934891	76673

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14276.28 Da
• Isoelectric Point: 7.8046

>T187816 WP_000854700.1 NZ_CP068799:c3906531-3906154 [Escherichia coli]
MKTLPDTHVREASRCLSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SPCTRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T187816 NZ_CP090028:1415486-1415839 [Serratia ureilytica]
GTGTGGGAAATTAAAACAACGGATGCATTTGATCATGGGTTCAGATCGCTCAGCGATGCCGATCGGGCCGGCGTACTTGC
CGCATTGATGGTCCTGCGGGAAAAGGGGCCGGGGCTGCCCAGACCCTATGTCGATATGGTTAAAGGCTCACGTTACAGCA
ATATGAAAGAGTTGCGCATTCAGTGCCGAGGCGAACCGTTGCGGGCATTCTTCGCTTTCGATCCGTACCGTATTGGCATT
GTGCTTTGCGCGGGCAATAAAGCCGGTAATGAAAAGCGTTTTTACGATCGGATGATCCAGGTTGCCGATCGGGAATTCAC
GAACTGGCTGAACACATTAAACGAGAAGGAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13422.27 Da
• Isoelectric Point: 6.3161

>AT187816 WP_001531802.1 NZ_CP068799:c3906989-3906621 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNSLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT187816 NZ_CP090028:1415844-1416146 [Serratia ureilytica]
ATGGGCAGAACGTTGGAACAGCTTATTGCGGATGAAAAGCCCGAGGTCGTGGCCAAAGCGCAGGCGCTGGCGGCGGACAT
CATGCTGAACATTCACCTTGCCGAGCTGCGTGAAAAAGTACAGAAAACGCAGGTTGAAATGGCGCAGGCGCTGGGGATCA
GGCAGCCGACCGTTGCCGTGATGGAAAAACCGGGCCGCGATCTGAAACTGTCTTCGCTTAAACGCTATGTTGAGGCGGCC
GGCGGCAAACTGCGCCTGGACATTGAACTGCCGGACGGCTCTCACTACGAGTTTGTGCTGTAA

Structures

Toxin

• AlphaFold DB: A0A454A1I2