Detailed information of TA system TA187813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3224592..3226312
• Replicon: chromosome
• Accession: NZ_CP068799
• Organism: Escherichia coli strain RIVM_C017887

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A7U9J2A7
• Locus tag: JNN46_RS15450
• Protein ID: WP_000312833.1
• Coordinates: 3224592..3225248 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: B7UP19
• Locus tag: JNN46_RS15455
• Protein ID: WP_000634203.1
• Coordinates: 3225245..3226312 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN46_RS15430	3219992..3220246	-	255	WP_154670580.1	hypothetical protein	-
JNN46_RS15435	3220528..3220767	+	240	WP_001431807.1	hypothetical protein	-
JNN46_RS15440	3220807..3223245	+	2439	WP_000985465.1	type I restriction-modification system subunit M	-
JNN46_RS15445	3223245..3224579	+	1335	WP_000228013.1	restriction endonuclease subunit S	-
JNN46_RS15450	3224592..3225248	+	657	WP_000312833.1	DUF4433 domain-containing protein	Toxin
JNN46_RS15455	3225245..3226312	+	1068	WP_000634203.1	macro domain-containing protein	Antitoxin
JNN46_RS15460	3226331..3229426	+	3096	WP_000108735.1	HsdR family type I site-specific deoxyribonuclease	-
JNN46_RS15465	3229426..3230142	+	717	WP_001122107.1	M48 family metallopeptidase	-
JNN46_RS15470	3230487..3231101	+	615	Protein_3036	IS3-like element IS600 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	aafC / aggD / iroC / iroD / iroE / iroN	3220807..3268489	47682

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25233.56 Da
• Isoelectric Point: 6.2145

>T187813 WP_000312833.1 NZ_CP068799:3224592-3225248 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Nucleotide

• Length: 657 bp

>T187813 NZ_CP090028:c364347-364057 [Serratia ureilytica]
ATGATTAAATCTTTCCGCGATCGCTATCTGGAGCAGTTCTATCTGGAAGGCAGGCGCAGCCGTCTTATCCCCGGCGCGCT
CGAGCGGCAGTTGGCCAGAAAGCTCGATATGTTAGCCGCCGCGCAGCAAGAGCGTGATTTGCATCACCCCACGGGTAACT
ATTACAAGCGGCTATCCGGGCCATTTCAGGGATGGTCGAGTCTGCGCGTCAATATGCACTGGCGGCTGATGTTCCAGTGG
CGGCATGATGCCGCCGAAAAAATTTACCTCGATCCTCACCAAGATACCTGA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40672.70 Da
• Isoelectric Point: 5.6927

>AT187813 WP_000634203.1 NZ_CP068799:3225245-3226312 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT187813 NZ_CP090028:c364042-363719 [Serratia ureilytica]
ATGTTGGAAACCACGACTCGCGAACCCACCACGGTGGGAGAAGTGCTGCAGGAAGAATATTTAACGCCGTTAGGCATCGG
CCAAGAAGAACTGGCAAAGGTATTGGGCGTCACCCGCGCCACCGTCAATCGCCTGTGCAACGGGCATCGACGCATGAGCG
TTGCCGAGGCCAATCTGTTTGCCGATCTGTTCGAAACGACCGCCGACTTTTGGCTAAACCTGCAGGCGGCACACGATCGC
TGGGAAGCCAGACAGGCCGATGCGCAGCGTGTGCGGCTGCGGCCAATCAAAGAAATACTGGGACGCAATATCACTCATGC
ATGA

Structures

Toxin

• AlphaFold DB: A0A7U9J2A7

Antitoxin

• AlphaFold DB: A0A7U9J2B9