Detailed information of TA system TA187810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2567203..2568791
• Replicon: chromosome
• Accession: NZ_CP068799
• Organism: Escherichia coli strain RIVM_C017887

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A192ENQ5
• Locus tag: JNN46_RS12130
• Protein ID: WP_001125432.1
• Coordinates: 2567469..2568791 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V0VAH2
• Locus tag: JNN46_RS12125
• Protein ID: WP_001304368.1
• Coordinates: 2567203..2567469 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN46_RS12110	2563571..2565022	+	1452	WP_000854642.1	tagaturonate reductase	-
JNN46_RS12115	2565219..2566133	+	915	WP_001298637.1	bestrophin family protein	-
JNN46_RS12120	2566137..2566895	-	759	WP_001286538.1	trans-aconitate 2-methyltransferase	-
JNN46_RS12125	2567203..2567469	+	267	WP_001304368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JNN46_RS12130	2567469..2568791	+	1323	WP_001125432.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JNN46_RS12135	2568967..2569659	-	693	WP_000382466.1	hypothetical protein	-
JNN46_RS12140	2569671..2569946	-	276	WP_000948310.1	hypothetical protein	-
JNN46_RS12145	2569948..2570088	-	141	WP_001335097.1	hypothetical protein	-
JNN46_RS12150	2570204..2570386	-	183	WP_001335098.1	hypothetical protein	-
JNN46_RS12155	2570556..2571608	+	1053	WP_000568119.1	hypothetical protein	-
JNN46_RS12160	2571620..2571937	+	318	WP_000073834.1	hypothetical protein	-
JNN46_RS12165	2572141..2572383	+	243	WP_000699011.1	phage capsid protein	-
JNN46_RS12170	2572430..2573710	+	1281	WP_000823306.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49297.02 Da
• Isoelectric Point: 8.6972

>T187810 WP_001125432.1 NZ_CP068799:2567469-2568791 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T187810 NZ_CP090026:c30163-29756 [Cereibacter azotoformans]
ATGCTGCGCTACATGCTCGACACGAACATCCTGATCTACACGATCAGGAACCGGCCTGGCGAGGTCAGGGCGAAATTCGA
GGCCCACCATGGGACGATGTGCATCTCGACCGTAACCGCGATGGAACTGATCTTCGGCGCGCAGAAATCGCAGGCCGTCG
AACGCAACATGGCGGCGGTCGAGGGACTTCTTGCGCGCCTCGACATCCTGGATCTCGACTTGGCGGCTGCCCAGCACGCT
GGGGAGATCCGGGCGCAGTTGGCGAGGGCAGGGACGCCCATCGGACCGTTCGACGTGATGATCGCCGGACACGCGCGATC
GAGGGGCCTGGTCCTCGTGAGCAACAACACGCGGGAATTCGAGCGCGTCGACGGCCTGAGGCTTGAGAACTGGGTCCAGC
CGTCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9957.31 Da
• Isoelectric Point: 4.9632

>AT187810 WP_001304368.1 NZ_CP068799:2567203-2567469 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Nucleotide

• Length: 267 bp

>AT187810 NZ_CP090026:c30372-30163 [Cereibacter azotoformans]
ATGAACAACCGGACGCAGGCCATCCGCCTGCCGAAATCGGTTGCCCTGCCGGAAGGCACGCGGAGCGTCGACATAGTCCG
GCTGGGCAGGGCTTGGCTCATCGTGCCGAGCCAGGACAGTTGGGCTCAGTGGTTCGATGAGGTCTCGTCGAGTGAAGACT
TCATGGAGCGCCGGGAGCAACCGGACGCTGATGAACGCGACGATCTCTGA

Structures

Toxin

• AlphaFold DB: A0A192ENQ5

Antitoxin

• AlphaFold DB: V0VAH2