Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1129394..1129977 Replicon chromosome
Accession NZ_CP068799
Organism Escherichia coli strain RIVM_C017887

Toxin (Protein)


Gene name mazF Uniprot ID S1PVD8
Locus tag JNN46_RS05305 Protein ID WP_000254749.1
Coordinates 1129642..1129977 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag JNN46_RS05300 Protein ID WP_000581937.1
Coordinates 1129394..1129642 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN46_RS05290 1125733..1127034 + 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
JNN46_RS05295 1127082..1129316 + 2235 WP_000226797.1 GTP diphosphokinase -
JNN46_RS05300 1129394..1129642 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JNN46_RS05305 1129642..1129977 + 336 WP_000254749.1 endoribonuclease MazF Toxin
JNN46_RS05310 1130049..1130840 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
JNN46_RS05315 1131068..1132705 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JNN46_RS05320 1132793..1134091 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12137.06 Da        Isoelectric Point: 8.7218

>T187799 WP_000254749.1 NZ_CP068799:1129642-1129977 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T187799 NZ_CP090005:c971288-970899 [Providencia rettgeri]
GTGGGAATTTATTTAACACCTGAATTTGAGGAAGAACGCCGGAACGCACGCATCAGTAATGCCATTTTGTGCAAAGCGGC
TAAAGCTATTTTTTCTGGTCTACCGGGCGATCCGCTAGGAAAATTCACCTACAAAAAGCGACTTGGCTTACCCGGAGTAG
GAGCGAAAGACGGTGCACGGTCAATTGTTTTTTTTAATAATGGCGAAAACCTATTTTTCTTTGATATGTATTTGAAAAGT
GAACTTTCCAAAAAGAAAGGAAAGGAGCTTGAAGCCGATGAAATTAATGCTTACTGCAATATCGCAAAAGATTTTATCGC
AATGAGTTCGGTAATGATAAAGACGTTGCTGACAGATAAAGAGCTGATTGAGGTGATTTGTGATGACTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT187799 WP_000581937.1 NZ_CP068799:1129394-1129642 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT187799 NZ_CP090005:c970906-970589 [Providencia rettgeri]
ATGACTGAACGACTGAAACGCATTCAGGGCATGGCTGAGCGCTACAATAAATTAGGCGTGGTGAGAAATGAAACCGTCGC
GGTTATCGATGCTGCGGTCGCGGCCCGTAAAATACCGGCTGTCCGTACCATGACCGGTAGCCAGATTAAAGAAGTTCGCA
CTCGCTACAACATGTCACAAGCCGCACTGGCGATGACGACCGGCATGTCTGTAGAGAGTGTGTCGAAGTGGGAGCGAAAC
GAGAGCAAGCCGAACAAGGCCGCTTTGCGGATCATTAACATCATTGATGATAAAGGGCCACAAGTGTTTATGTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PVD8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References