Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5364341..5364966 Replicon chromosome
Accession NZ_CP068798
Organism Klebsiella pneumoniae strain RIVM_C015043

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JNN64_RS26255 Protein ID WP_002882817.1
Coordinates 5364341..5364724 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JNN64_RS26260 Protein ID WP_004150355.1
Coordinates 5364724..5364966 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN64_RS26240 5361707..5362609 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JNN64_RS26245 5362606..5363241 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JNN64_RS26250 5363238..5364167 + 930 WP_025368581.1 formate dehydrogenase accessory protein FdhE -
JNN64_RS26255 5364341..5364724 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JNN64_RS26260 5364724..5364966 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JNN64_RS26265 5365171..5366088 + 918 WP_025368582.1 alpha/beta hydrolase -
JNN64_RS26270 5366103..5367044 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JNN64_RS26275 5367089..5367526 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JNN64_RS26280 5367523..5368383 - 861 WP_004146232.1 virulence factor BrkB family protein -
JNN64_RS26285 5368377..5368976 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T187793 WP_002882817.1 NZ_CP068798:c5364724-5364341 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T187793 NZ_CP090001:c2073752-2073543 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT187793 WP_004150355.1 NZ_CP068798:c5364966-5364724 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT187793 NZ_CP090001:c2074057-2073764 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References