Detailed information of TA system TA187776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 324326..324912
• Replicon: chromosome
• Accession: NZ_CP068798
• Organism: Klebsiella pneumoniae strain RIVM_C015043

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A9J6Y968
• Locus tag: JNN64_RS01510
• Protein ID: WP_025367836.1
• Coordinates: 324544..324912 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: JNN64_RS01505
• Protein ID: WP_004174006.1
• Coordinates: 324326..324547 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN64_RS01485	320483..321409	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
JNN64_RS01490	321406..322683	+	1278	WP_004188292.1	branched chain amino acid ABC transporter permease LivM	-
JNN64_RS01495	322680..323447	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
JNN64_RS01500	323449..324162	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
JNN64_RS01505	324326..324547	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JNN64_RS01510	324544..324912	+	369	WP_025367836.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
JNN64_RS01515	325185..326501	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
JNN64_RS01520	326608..327495	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
JNN64_RS01525	327492..328337	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
JNN64_RS01530	328339..329409	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	319312..330146	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13449.86 Da
• Isoelectric Point: 7.2790

>T187776 WP_025367836.1 NZ_CP068798:324544-324912 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIECEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLLG

Nucleotide

• Length: 369 bp

>T187776 NZ_CP089997:283903-284253 [Cytobacillus spongiae]
TTGAATGTAAAACGTGGTGACGTTTACTTTGCAGACCTATCCCCTGTTGTTGGTTCTGAACAAGGCGGAGTACGCCCGGT
ACTTGTTATCCAAAACGACATCGGGAATCGGTTTAGTCCAACAGTAATTGTTGCGGCCATTACCGCGCAAATACAAAAAG
CGAAGCTGCCGACTCACGTTGAAATTGATGCGAAGAAATATGGTTTTGAACGGGATTCAGTGATCTTATTAGAACAGATT
CGTACAATTGACAAACAACGTTTAACCGATAAAATTACTCATCTCGATGACGAAATGATGGAAAAGGTAAACGAAGCCTT
GCAGGTAAGCTTAGGTCTTATAGATTTTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT187776 WP_004174006.1 NZ_CP068798:324326-324547 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT187776 NZ_CP089997:283605-283898 [Cytobacillus spongiae]
GTGTCTGAATCCAGCGCAACAACAGAAATCATGGTAGAATTACCGCAACAACTTTTGTCTGAACTAGATGTTTATATTAG
CTCAGAACAGGTCAATCGCAATGAATTTATCAAACATGCGACGCAAAAGTATTTACGTGAGAGAAAGAAAAGACAAATTC
GCGAATCCATGAGACGGGGCTATATGGAAATGGCGAAAATTAATCTGATGATTGCATCTGAAGCTTTTCAAGCAGAATAC
GAGGCAGAAAACACAGTTACTAACAGGGTTGAACGTCTAGTTAGCGGGGGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7