Detailed information of TA system TA187735

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 830430..831265
• Replicon: chromosome
• Accession: NZ_CP068796
• Organism: Escherichia coli strain RIVM_C012087

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0A1A9A5
• Locus tag: JNN38_RS03925
• Protein ID: WP_001564063.1
• Coordinates: 830430..830807 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0K5N986
• Locus tag: JNN38_RS03930
• Protein ID: WP_021553056.1
• Coordinates: 830897..831265 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN38_RS03895	825558..826706	-	1149	WP_000905920.1	capsule polysaccharide export inner-membrane protein KpsE	-
JNN38_RS03900	826778..827761	-	984	WP_001296394.1	KpsF/GutQ family sugar-phosphate isomerase	-
JNN38_RS03905	828571..828741	-	171	Protein_766	IS110 family transposase	-
JNN38_RS03910	829084..829926	-	843	Protein_767	DUF4942 domain-containing protein	-
JNN38_RS03915	830011..830205	-	195	WP_203003946.1	hypothetical protein	-
JNN38_RS03920	830284..830433	-	150	Protein_769	hypothetical protein	-
JNN38_RS03925	830430..830807	-	378	WP_001564063.1	TA system toxin CbtA family protein	Toxin
JNN38_RS03930	830897..831265	-	369	WP_021553056.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN38_RS03935	831428..831649	-	222	WP_000692345.1	DUF987 domain-containing protein	-
JNN38_RS03940	831714..832190	-	477	WP_021553055.1	RadC family protein	-
JNN38_RS03945	832206..832685	-	480	WP_001564060.1	antirestriction protein	-
JNN38_RS03950	832951..833769	-	819	WP_001564059.1	DUF945 domain-containing protein	-
JNN38_RS03955	833859..834092	-	234	WP_001278293.1	DUF905 family protein	-
JNN38_RS03960	834098..834775	-	678	WP_001564058.1	hypothetical protein	-
JNN38_RS03965	834926..835606	-	681	WP_001278649.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / kpsM / kpsT / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / papX	725589..897916	172327

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14094.09 Da
• Isoelectric Point: 7.8045

>T187735 WP_001564063.1 NZ_CP068796:c830807-830430 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T187735 NZ_CP089980:c629917-629657 [Fulvivirga sp. SW1-E11]
GTGAATGTTATTTTCTCACATAACGCATGGGAAGATTACACCTCTTGGTTAAAGGAAGATAGAAAGATATTGAAGAAAAT
AAATGACTTAGTCAAGGATATCAAGAGAACCCCTTTGGAAGGAATTGGTAAGCCAGAACCCTTAAAATATGAGCTTTCTG
GCTATTGGTCGCGTAGAATCGACCGAGAACATAGATTAGTATATCAGGTTTCAGGTGCAGATTTGATCATTTTTAGCTGT
AAATATCACTATGATAAATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13615.39 Da
• Isoelectric Point: 7.0264

>AT187735 WP_021553056.1 NZ_CP068796:c831265-830897 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT187735 NZ_CP089980:c630171-629917 [Fulvivirga sp. SW1-E11]
ATGATAGCAGCAAATTATACAGAGTTTAGGACTAACCTTAAGAAGTATCTTGATGATGTGGAGCAAAATCAAGAAACATT
GATTGTTAAAAGAGGGACGGGAAATGGTACCGTAATGATTTCACTTGACGAATATAATTCTTTAATGGAAACAGTTCATT
TGTTGAGCTCAAAGAAAAATGCTGATAGACTGTTTGAATCGATACAGCAGATAAAAGATGGAAAAGTAGTTAAACGAGGG
TTGATTGAGGGTTAG

Structures

Toxin

• AlphaFold DB: A0A0A1A9A5

Antitoxin

• AlphaFold DB: A0A0K5N986