Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4416369..4416866 Replicon chromosome
Accession NZ_CP068795
Organism Escherichia coli strain RIVM_C011532

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JNN54_RS21380 Protein ID WP_001173343.1
Coordinates 4416369..4416542 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JNN54_RS21385 Protein ID WP_000398619.1
Coordinates 4416570..4416866 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN54_RS21370 4413034..4414491 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
JNN54_RS21375 4414728..4416245 + 1518 WP_001295074.1 lysine--tRNA ligase -
JNN54_RS21380 4416369..4416542 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JNN54_RS21385 4416570..4416866 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JNN54_RS21390 4417093..4417365 - 273 WP_000405647.1 N-acetyltransferase -
JNN54_RS21395 4417377..4417607 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
JNN54_RS21400 4417788..4419419 + 1632 WP_001216477.1 sensor histidine kinase -
JNN54_RS21405 4419416..4420135 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T187731 WP_001173343.1 NZ_CP068795:c4416542-4416369 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T187731 NZ_CP089979:c82941-82687 [Fulvivirga sp. W9P-11]
ATGGAAGTAACTAATTATTCTAACTTCAGGCAAAACCTAAAGAGCTTTTTAGATAAAGTCTTCGTATCTCACGATCCACT
ATACGTATCTCGAGCTAATGGTGAAGATGTGGTGGTCATGTCGAAATCTGATTATGAAAGTATTCAGGAGACACTTTATC
TTCTAAGTAGCCCAAAAAATGCCGATCGGTTGGCTAAAGGCATAGAAGAATATGAGCATGGAAAAGGCTTGAAAAAAGAC
CTTATTGAAGATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT187731 WP_000398619.1 NZ_CP068795:c4416866-4416570 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT187731 NZ_CP089979:c82687-82433 [Fulvivirga sp. W9P-11]
ATGGAAATTATTTTTCTGTCTCCCGCTTGGGAGGATTATATCTACTGGCAGCAAACCGATAAGAAAATCTTAAGGAAAAT
TAATGAGCTCATCAGGCAATGCCAACGGACACCTTTTGAAGGCATCGGAAAACCTGAGCCTTTAAGGGGCAATCTTTCCG
GTTGGTGGTCACGGCGAATAAACCATGAACATCGATTAGTTTATAAGGTGGAAAATGAAAGTCTTTATATTTTACAGTGT
AGAAGGCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References