Detailed information of TA system TA187729

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4217799..4218634
• Replicon: chromosome
• Accession: NZ_CP068795
• Organism: Escherichia coli strain RIVM_C011532

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2S4A272
• Locus tag: JNN54_RS20385
• Protein ID: WP_001094443.1
• Coordinates: 4217799..4218176 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7LDN9
• Locus tag: JNN54_RS20390
• Protein ID: WP_001285607.1
• Coordinates: 4218266..4218634 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN54_RS20355	4213012..4214118	+	1107	WP_001353974.1	N-acetylneuraminate epimerase	-
JNN54_RS20360	4214183..4215163	+	981	WP_001295601.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
JNN54_RS20365	4215171..4215821	-	651	WP_001037966.1	HNH endonuclease	-
JNN54_RS20370	4216866..4217012	-	147	WP_001290178.1	hypothetical protein	-
JNN54_RS20375	4217097..4217294	-	198	WP_001327226.1	DUF957 domain-containing protein	-
JNN54_RS20380	4217314..4217802	-	489	WP_000761685.1	hypothetical protein	-
JNN54_RS20385	4217799..4218176	-	378	WP_001094443.1	TA system toxin CbtA family protein	Toxin
JNN54_RS20390	4218266..4218634	-	369	WP_001285607.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN54_RS20395	4218714..4218935	-	222	WP_000692350.1	DUF987 domain-containing protein	-
JNN54_RS20400	4219022..4219498	-	477	WP_001186165.1	RadC family protein	-
JNN54_RS20405	4219513..4219998	-	486	WP_000206664.1	antirestriction protein	-
JNN54_RS20410	4220090..4220908	-	819	WP_001175163.1	DUF945 domain-containing protein	-
JNN54_RS20415	4220998..4221231	-	234	WP_001278287.1	DUF905 family protein	-
JNN54_RS20420	4221237..4221914	-	678	WP_001519094.1	hypothetical protein	-
JNN54_RS20425	4222062..4222742	-	681	WP_203134127.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimE / fimB	4208399..4243915	35516

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14058.97 Da
• Isoelectric Point: 7.3523

>T187729 WP_001094443.1 NZ_CP068795:c4218176-4217799 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T187729 NZ_CP089977:752532-752921 [Moraxella nasicaprae]
ATGTTAGATACAAACATTGTTATCCATATCATCAAACAAAAGCCAGCAAAGTTGTTGGGTAAATTTAATCAGTATGCTGG
CAGGATTTGCATTAGTGCTATTACAGCGTCCGAACTATGGGCAGGCGTGTATCACAGTCAAAGAGTGTATGAAAATGGCA
AAATTTTGGCGGATTTTTTGTCAAGGATTGATGTGCTACCCTACAAGCCTGATTGTTGCCAGTACTATGGGGAAAATTAT
GCACAGCTTGCCAAAGATGGGCGATTGATTAGCGAAAATGATTTACACATTGCCAGCCACGCCAAATCACTAGGCTTAAT
TCTAGTAACCAACAACACCAAAGAATTTGCACGAGTACAAGGGCTTGAATTGCAGGACTGGCTGGAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13689.53 Da
• Isoelectric Point: 6.4669

>AT187729 WP_001285607.1 NZ_CP068795:c4218634-4218266 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT187729 NZ_CP089977:752272-752505 [Moraxella nasicaprae]
ATGCCCATAACCACCGTATTTATGAACAATCGCACCCAAGCCGTACGCATTCCCAGCGGTTTAAAATTTAATGATGATAT
TAAGCAAGTCATCATCAGAAAAGTAGGTAAAGAGCTAATTATTAGCCCCCAAGATGGCGTATGGGACAGCTTTTTTTTAT
CCGATGAGCGTGTATCAGATGATTTTGACCGCCCCACGCCTGCAATGCCATCACAACGAGCGTCATTTGATTAA

Structures

Toxin

• AlphaFold DB: A0A2S4A272

Antitoxin

• AlphaFold DB: A0A1M0JLU8