Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3589000..3589618 Replicon chromosome
Accession NZ_CP068795
Organism Escherichia coli strain RIVM_C011532

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JNN54_RS17345 Protein ID WP_001291435.1
Coordinates 3589400..3589618 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JNN54_RS17340 Protein ID WP_000344800.1
Coordinates 3589000..3589374 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN54_RS17330 3584089..3585282 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JNN54_RS17335 3585305..3588454 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
JNN54_RS17340 3589000..3589374 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JNN54_RS17345 3589400..3589618 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JNN54_RS17350 3589790..3590341 + 552 WP_000102564.1 maltose O-acetyltransferase -
JNN54_RS17355 3590457..3590927 + 471 WP_000136192.1 YlaC family protein -
JNN54_RS17360 3591091..3592641 + 1551 WP_064221513.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JNN54_RS17365 3592683..3593036 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JNN54_RS17375 3593415..3593726 + 312 WP_000409911.1 MGMT family protein -
JNN54_RS17380 3593757..3594329 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T187726 WP_001291435.1 NZ_CP068795:3589400-3589618 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T187726 NZ_CP089976:c18331-17939 [Moraxella nasovis]
ATGAAATACCTGCTTGACACCAACATCATCATTGCCCTACAAAAGCAAAATCCTGTGCTTATCGCACGACTAAAACAACG
TAAAGTGAGCGATTTTGCGTTGTCGGATATTGTGCTGTTCGAGCTGTATTATGGGGCATATCATAGTCAAAAACAGCGTG
AAAATTTGGCAAAAATTGACAAATTACCCTTTGTAAGATTGCCTTTTGATGATAAAGATGCTCTATGTGCTGGCAAGGTG
CGAGCAGACCTACAAAGACAGGGGCAAGTGATCGGGGCGTATGACATGCTAATAGCAGGACAAGCGTTATCTAAAAATCT
AACCTTGATTACGCATAATGTCAAAGAGTTTAGCCGAGTTGACGGCTTAAAATGGCAAAACTGGTTAGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT187726 WP_000344800.1 NZ_CP068795:3589000-3589374 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT187726 NZ_CP089976:c18561-18328 [Moraxella nasovis]
ATGATTACCGCACGCCTTTTTTATAGTGGCAATTCACAAGCCATTCGCTTACCCAAAGAATTTCGCTTTGATGGCGATAA
AGTACAGTTAAAAAAACACGGCAACCAACTCATCATCACGCCCATCATAGATGATTGGGGCTGGCTGGATGATGTTTCGT
CCTTTGATGATGACATGGAGCAAGCAGTCGCAATGCTAAAAGACGACCCCATCAGCAAGCGAGATTTTGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References