Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2474435..2475073 Replicon chromosome
Accession NZ_CP068795
Organism Escherichia coli strain RIVM_C011532

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JNN54_RS11965 Protein ID WP_000813794.1
Coordinates 2474897..2475073 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JNN54_RS11960 Protein ID WP_001270286.1
Coordinates 2474435..2474851 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN54_RS11940 2469587..2470528 - 942 WP_001251313.1 ABC transporter permease -
JNN54_RS11945 2470529..2471542 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
JNN54_RS11950 2471560..2472705 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
JNN54_RS11955 2472950..2474356 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
JNN54_RS11960 2474435..2474851 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JNN54_RS11965 2474897..2475073 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JNN54_RS11970 2475295..2475525 + 231 WP_000494244.1 YncJ family protein -
JNN54_RS11975 2475617..2477578 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JNN54_RS11980 2477651..2478187 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
JNN54_RS11985 2478240..2479454 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T187724 WP_000813794.1 NZ_CP068795:c2475073-2474897 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T187724 NZ_CP089974:c1768516-1768325 [Moraxella sp. ZY171148]
ATGGCAAGTAGTAGAGAAGTCATTAAAATCATCAAAGCTGATGGTTGGTTTCAAATTAAGGCAAATGCTGGCAGTCATAA
GCAGTTCAAGCACCCAACCAAAAAAGGACGAGTAACTGTACCAGACCCTAAAAAAGACCTTGATAAAGAAACCGTCAAAA
GCATTATGATACAAGCAGGGCTAAGCGATTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT187724 WP_001270286.1 NZ_CP068795:c2474851-2474435 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT187724 NZ_CP089974:c1768292-1767888 [Moraxella sp. ZY171148]
ATGTTATATCCCATCGCTGTAAAACTAGGCAATGAACAAACCGCTCACGCCATTTTTTTTCCAGACATTCCGCATTTGGT
ATCTGCCTGTGATGAGCTTGATGACTTGTTGCATTGTGCAATCGAATGTATTGATTTGCATTTTAGTGGATTGGCAGAAG
ATGGCGAGGATATTCCTTTGCCCACAAAATTTAGCACGCATCAACAAAACCCCAAATATACAGGCTATATGTGGGCATGG
GTTGATATTGACCTAGCCAAGTACAATACTAAAACCCATAAAATCAATATCACTTTGCCACAATTTTTAATCAATCAAAT
TGATGAAAAAGTAGCAAGTCATAAACATTTATATAAAAGCCGCAGCAACTATCTAGCACAGCTTGCCAGTCGTGATTTGG
CATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References