Detailed information of TA system TA187714

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1883920..1884755
• Replicon: chromosome
• Accession: NZ_CP068795
• Organism: Escherichia coli strain RIVM_C011532

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JNN54_RS08940
• Protein ID: WP_064221650.1
• Coordinates: 1883920..1884297 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: JNN54_RS08945
• Protein ID: WP_001295723.1
• Coordinates: 1884387..1884755 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN54_RS08905	1879440..1879913	+	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-
JNN54_RS08910	1880111..1881169	+	1059	WP_001200895.1	FUSC family protein	-
JNN54_RS08915	1881341..1881670	+	330	WP_000450409.1	DUF496 family protein	-
JNN54_RS08920	1881771..1881953	-	183	WP_024174802.1	ethanolamine utilization protein	-
JNN54_RS08925	1882407..1882778	-	372	WP_001295631.1	IS110 family transposase	-
JNN54_RS08930	1883594..1883707	-	114	WP_032140621.1	DUF957 domain-containing protein	-
JNN54_RS08935	1883720..1883923	-	204	WP_000761705.1	hypothetical protein	-
JNN54_RS08940	1883920..1884297	-	378	WP_064221650.1	TA system toxin CbtA family protein	Toxin
JNN54_RS08945	1884387..1884755	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN54_RS08950	1884918..1885139	-	222	WP_000692345.1	DUF987 domain-containing protein	-
JNN54_RS08955	1885202..1885678	-	477	WP_147659663.1	RadC family protein	-
JNN54_RS08960	1885694..1886179	-	486	WP_203134131.1	antirestriction protein	-
JNN54_RS08965	1886234..1887052	-	819	WP_072649824.1	DUF945 domain-containing protein	-
JNN54_RS08970	1887207..1887365	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14150.14 Da
• Isoelectric Point: 7.3249

>T187714 WP_064221650.1 NZ_CP068795:c1884297-1883920 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTQLARLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T187714 NZ_CP089961:20781-21263 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT187714 WP_001295723.1 NZ_CP068795:c1884755-1884387 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT187714 NZ_CP089961:20527-20793 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: S1NM52