Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1097567..1098150 Replicon chromosome
Accession NZ_CP068795
Organism Escherichia coli strain RIVM_C011532

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag JNN54_RS05330 Protein ID WP_061349013.1
Coordinates 1097815..1098150 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag JNN54_RS05325 Protein ID WP_000581937.1
Coordinates 1097567..1097815 (+) Length 83 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN54_RS05315 1093906..1095207 + 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
JNN54_RS05320 1095255..1097489 + 2235 WP_000226815.1 GTP pyrophosphokinase -
JNN54_RS05325 1097567..1097815 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JNN54_RS05330 1097815..1098150 + 336 WP_061349013.1 endoribonuclease MazF Toxin
JNN54_RS05335 1098221..1099012 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
JNN54_RS05340 1099240..1100877 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JNN54_RS05345 1100965..1102263 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-111)

Antitoxin

(7-51)


Sequences


Toxin        


Download         Length: 112 a.a.        Molecular weight: 12112.07 Da        Isoelectric Point: 8.2618

>T187712 WP_061349013.1 NZ_CP068795:1097815-1098150 [Escherichia coli]
MVSRYVPDMGDLIWIDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T187712 NZ_CP089960:2235-2705 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT187712 WP_000581937.1 NZ_CP068795:1097567-1097815 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT187712 NZ_CP089960:1795-2238 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References