Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 7395..7996 Replicon plasmid p5
Accession NZ_CP068770
Organism Lactiplantibacillus plantarum strain S58

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag JMO19_RS15680 Protein ID WP_001748110.1
Coordinates 7652..7996 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag JMO19_RS15675 Protein ID WP_001748109.1
Coordinates 7395..7658 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMO19_RS15660 2453..3373 + 921 WP_016511843.1 IS30 family transposase -
JMO19_RS15665 3629..4189 + 561 WP_003643342.1 hypothetical protein -
JMO19_RS15670 6722..7309 - 588 WP_003646144.1 site-specific integrase -
JMO19_RS15675 7395..7658 + 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JMO19_RS15680 7652..7996 + 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JMO19_RS15685 8230..9017 + 788 WP_128383814.1 IS5 family transposase -
JMO19_RS15690 9107..9961 - 855 WP_003645004.1 transporter substrate-binding domain-containing protein -
JMO19_RS15695 9982..10722 - 741 WP_003645003.1 amino acid ABC transporter ATP-binding protein -
JMO19_RS15700 10715..11404 - 690 WP_003645002.1 amino acid ABC transporter permease -
JMO19_RS15705 11385..12047 - 663 WP_003645001.1 amino acid ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..15331 15331


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T187684 WP_001748110.1 NZ_CP068770:7652-7996 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T187684 NZ_CP089942:4700204-4700494 [Erwinia tracheiphila]
CTGCCGGGTGATCCACTTGGAAAATTCACCTTTAAAAAGCGCCTCGGCTTGCCCGGAGTAGGAGCCAGAGATGGAGCACG
ATCAATCGTTTTTTTCAATGATGGCGACAACATCTTCTTTTTTGATATATATCTGAAAAGTGGGCTTTCAAAAAAGAAAG
GAAAAGAGCTTGAAGATGATGAAATTGATGCTTACTGCAATATCGCAAAAGATTTTATCTCAATGAGTCCGGCAACTATA
AATAAGCTTCTGTCTGATAAAGAACTGATCGAGGTAAATTGTGATGACTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT187684 WP_001748109.1 NZ_CP068770:7395-7658 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT187684 NZ_CP089942:4700487-4700804 [Erwinia tracheiphila]
ATGACTGAACGTCTGAAACGCATTCAAGGTATGGCTGAACGCTATAACAAACTAGGCGTGGTAAAAGATGAAACCGTAGC
TGCAATTGATGCAGCGGTCGCAGCCCGTAATATCCCGGCGATCCGAGTTATGACCGGAAGCCAGATAAAGGAAGTCCGCA
CTCGCTACAACATGTCGCAAGCCGCATTAGCTATAACAACCGGCATGTCTGTAGAAAGCGTCTCCAAATGGGAGCGAAAC
GAAAGCAAACCGAATAAGGCTGCTCTGCGGATCATCAACATCATCGATGCTAAAGGGCCACAGGTGTTCATGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References