Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 252881..253523 Replicon chromosome
Accession NZ_CP068724
Organism Bacillus cereus strain AFA01

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag JM957_RS01410 Protein ID WP_000635963.1
Coordinates 253173..253523 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag JM957_RS01405 Protein ID WP_000004570.1
Coordinates 252881..253168 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JM957_RS01380 248198..249160 + 963 WP_000961165.1 UV DNA damage repair endonuclease UvsE -
JM957_RS01385 249153..249725 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
JM957_RS01390 249819..250178 + 360 WP_000583416.1 holo-ACP synthase -
JM957_RS01395 250335..251285 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
JM957_RS01400 251403..252572 + 1170 WP_000390615.1 alanine racemase -
JM957_RS01405 252881..253168 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
JM957_RS01410 253173..253523 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JM957_RS01415 253591..255759 + 2169 WP_000426242.1 RNA-binding transcriptional accessory protein -
JM957_RS01420 255817..255933 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
JM957_RS01425 256129..256587 + 459 WP_000344234.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T187675 WP_000635963.1 NZ_CP068724:253173-253523 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T187675 NZ_CP089942:312456-312731 [Erwinia tracheiphila]
ATGAATCAAGCCGTTAAAAATCTCAGGAAAAAGCAGCGTGATACCCTGGAACAGATATTCAGAACGTCGCCACCGTCAGG
ACTGAAGTGGTCAGCGGTGGAGTCCCTGATCGGCGCTCTGTGCGGCAAAATTAAAGAGGGTAGCGGATCGCGCGTGCGTT
TTTTGCTTAACGGCAGTATTGCCCGTTTCCACCGTCCGCACCCGTCACCTGATACCGATAAAGGCGCAGTCGTCAGCCTG
CGCGAATGGTTAGAAAGCACCGGAGTGAAACCATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT187675 WP_000004570.1 NZ_CP068724:252881-253168 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT187675 NZ_CP089942:312728-313087 [Erwinia tracheiphila]
ATGACAAAACCAGCCACCCCAAATATGATCGTTATTGCCGGGCAGCCAGCGGTTGTCAGCTACGTGCCGGAAATTAATAT
GTTTCGCGGGCGCTTTCTGGGGCTGACAGGCTACTGTGACTTTGTTTCTGACAGTATTCAGGGACTGAACAAAGAAGGCG
ATATTTCGCTTCGTGAGTATCTGGAAGATTGTAGTGCGGAAGGTATAGAACCTTACGCCCGCCAGGAAAAAACCAGGACA
TTCACGCTTCGCTACCCTGAATCTTTCGGCGAACGCCTGAATCAGGCAGCCGCAGAAAATGAAGTATCGGTGAATGCGTT
CATTGTTGAGACGTTAAACGAGCGCATGAAGCATGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References