Detailed information of TA system TA187672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2428266..2429244
• Replicon: chromosome
• Accession: NZ_CP068717
• Organism: Bacillus cereus strain CH

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: JM955_RS12345
• Protein ID: WP_000624977.1
• Coordinates: 2428266..2429003 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: JM955_RS12350
• Protein ID: WP_000237819.1
• Coordinates: 2429116..2429244 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JM955_RS12325	2423908..2424690	+	783	WP_063547282.1	class I SAM-dependent methyltransferase	-
JM955_RS12330	2424848..2426533	-	1686	WP_063547283.1	alpha-keto acid decarboxylase family protein	-
JM955_RS12335	2426641..2427123	+	483	WP_000191889.1	MarR family winged helix-turn-helix transcriptional regulator	-
JM955_RS12340	2427290..2428027	+	738	WP_063547284.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
JM955_RS12345	2428266..2429003	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
JM955_RS12350	2429116..2429244	+	129	WP_000237819.1	hypothetical protein	Antitoxin
JM955_RS12355	2429317..2429493	+	177	WP_063547285.1	hypothetical protein	-
JM955_RS12360	2429537..2429812	-	276	WP_063547286.1	hypothetical protein	-
JM955_RS12365	2429839..2430633	-	795	WP_176328690.1	hypothetical protein	-
JM955_RS12370	2430694..2431674	-	981	WP_063547287.1	WXG100 family type VII secretion target	-
JM955_RS12375	2432005..2432181	+	177	WP_000852629.1	stage II sporulation protein SB	-
JM955_RS12380	2432198..2432587	-	390	WP_000713869.1	YxeA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T187672 WP_000624977.1 NZ_CP068717:2428266-2429003 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T187672 NZ_CP089940:4860081-4860377 [Erwinia tracheiphila]
ATGATCGAGATAAAACGGACGCCGGAAGTTGAAAAATGGCTCAAGTCTTTGAAAGACAGAACGGCAAAAGCAAAAATATT
AATGCGACTTGAACGGATGGAGGACGGGAATTTTGGAGATGTAGAGCCTATTGGTGATGGCTTATCTGAATTGAGAGTTC
ATCAGGGTAAAGGCTACCGTGTGTATTTTGGGAACAAAAATAATAAAATTATTCTCTTGCTTTGCGGTGGAGATAAAAGC
ACACAGCAGGCAGATATCAGAAAGGCAAAACAATTAGCCAAACAGTGGGGTTTTTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT187672 WP_000237819.1 NZ_CP068717:2429116-2429244 [Bacillus cereus]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT187672 NZ_CP089940:4860379-4860663 [Erwinia tracheiphila]
ATGAAAGAAGAACTTAAAAAATTTGATGTTGTTGAATTTCTTGAAGATGAAGAAGATATTCAGGAATATCTTAATGCTGC
AATTGAAGAAGATGATACAAAATACCTTTTTAAAGCATTAGGTAACATAGCCAGAGCAAGAAACATGAGTCAGCTATCTA
AAGAAGTTGGCATGTCCAGAGAGGGAATTTACAAGGCGTTATCCGGTGAAGGGAATCCATCATTCCAGACTGTTTCTAAA
ATCACAAAAGCGCTAGGCTTAAAACTTCACTTCACAGGGGCGTAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2