187655

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 312033..312943 Replicon plasmid p1-pV-hybrid-1
Accession NZ_CP068707
Organism Escherichia coli strain J53-p1-pV-hybrid-1

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag JK375_RS24240 Protein ID WP_004181896.1
Coordinates 312033..312503 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag JK375_RS24245 Protein ID WP_004181895.1
Coordinates 312500..312943 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JK375_RS24220 308071..309039 - 969 WP_176691886.1 IS5-like element IS903B family transposase -
JK375_RS24225 309681..309839 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
JK375_RS24230 309911..311092 + 1182 WP_004883380.1 IS481 family transposase -
JK375_RS24235 311663..311923 + 261 WP_004026352.1 hypothetical protein -
JK375_RS24240 312033..312503 - 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
JK375_RS24245 312500..312943 - 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
JK375_RS24250 313044..314315 - 1272 WP_065810726.1 translesion error-prone DNA polymerase V subunit UmuC -
JK375_RS24255 314315..314737 - 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
JK375_RS24260 314823..316310 - 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
JK375_RS24265 317002..317571 - 570 Protein_333 transposase -
JK375_RS24270 317573..317788 + 216 Protein_334 IS200/IS605 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(7'')-Ia rmpA / iutA / iucD / iucC / iucB / iucA / iroN / iroD / iroC / iroB 1..324396 324396
- flank IS/Tn - - 317002..317388 386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T187655 WP_004181896.1 NZ_CP068707:c312503-312033 [Escherichia coli]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T187655 NZ_CP089934:3238433-3238612 [Erwinia tracheiphila]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTAAGAGTAAAAGGCAGTCATCACCAGTT
CAAGCACCCAACGAAAAAAGGGCTGGTAACGGTAAAACATTCACAGAAGGACATACCGCAACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTTTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT187655 WP_004181895.1 NZ_CP068707:c312943-312500 [Escherichia coli]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT187655 NZ_CP089934:3238635-3239039 [Erwinia tracheiphila]
ATGTTATACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTGCCTGATATTCCAGGCTGCTTCTCTGG
CGGAGACAACTATCAGGATGCGATTGAGAGCGTCCGCGAGGCTATTGACGCACATATCGAATTACTTGTGGAAGATGGCG
AAGGGTTGCCGGAGGCGACCAGCGTGGAAAACTGGCTGGCCGATCCCGATTATGTAGACGTGGTATGGGCGCTGGTTGAT
GTGGACGTTACCCGATTAATGGGGAAAGCGGAAAAGATAAACGTCACGCTCCCTTCGCTGCTTATCCGCCGTATCGACCA
ATTTGTGGCCGCGCATCCTGAATACGGTAGCCGTTCCGGTTTCCTATCCCGTGTCGCTGCAGATAAAGTCATTGGCAGGA
TCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References