Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 233943..234853 Replicon plasmid p1-pV-hybrid-1
Accession NZ_CP068707
Organism Escherichia coli strain J53-p1-pV-hybrid-1

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag JK375_RS23865 Protein ID WP_004026354.1
Coordinates 234383..234853 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag JK375_RS23860 Protein ID WP_004026357.1
Coordinates 233943..234386 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JK375_RS23830 228961..229482 + 522 WP_004213635.1 sigma-70 family RNA polymerase sigma factor -
JK375_RS23835 229479..230432 + 954 Protein_247 fec operon regulator FecR -
JK375_RS23840 230519..232645 + 2127 WP_004213640.1 TonB-dependent receptor family protein -
JK375_RS23845 232720..232875 + 156 WP_004213642.1 hypothetical protein -
JK375_RS23850 233020..233241 + 222 WP_004213643.1 DUF2188 domain-containing protein -
JK375_RS23855 233339..233842 + 504 Protein_251 DUF4113 domain-containing protein -
JK375_RS23860 233943..234386 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
JK375_RS23865 234383..234853 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
JK375_RS23870 235002..235699 + 698 Protein_254 IS1 family transposase -
JK375_RS23875 237085..238034 - 950 Protein_255 DUF1173 family protein -
JK375_RS23880 238212..238706 + 495 WP_004212794.1 thermonuclease family protein -
JK375_RS23885 239043..239441 + 399 WP_004212796.1 H-NS histone family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(7'')-Ia rmpA / iutA / iucD / iucC / iucB / iucA / iroN / iroD / iroC / iroB 1..324396 324396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T187654 WP_004026354.1 NZ_CP068707:234383-234853 [Escherichia coli]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T187654 NZ_CP089934:3210514-3210693 [Erwinia tracheiphila]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTAAAAGGCAGTCATCACCAGTT
TAAACATCCAATGAAAAAGGGGCTAGTAACGGTAAAACATCCACAAAAGGACATACCGCTACCAACGCTAAAAAGCATCA
AAAAACAGGCGGGGCTTTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT187654 WP_004026357.1 NZ_CP068707:233943-234386 [Escherichia coli]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT187654 NZ_CP089934:3210715-3211110 [Erwinia tracheiphila]
ATGTTATATCCCGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTGCCTGATATTCCTGGCTGCTTCTCTGG
CGGCGACAACTATCAGGATGCGATTGAGAGCGTGCGTGAAGCTATCGACGCACATATCGAATTACTGGTAGAAGATGGTG
AATCGGTGCCGGAGGCGACCAACGTGGAAAACTGGCTGGCCGATCCCGATTATGTTGGCGTGGTATGGGCGCTGGTTGAT
GTGGACGTCACCCGGTTAATGGGTAAAGCGGAAAAGATCAACGTCACGCTCCCTTCGCTGCTTATCCGCCGTATCGACCA
ATTTGTGGCCGCGCATCCTGAATACGGTAGCCGTTCCGGTTTCCTGTCCCGTGTCGCTGCAGATAAAGTGTACTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References