Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 177931..178601 Replicon plasmid p1-pV-hybrid-1
Accession NZ_CP068707
Organism Escherichia coli strain J53-p1-pV-hybrid-1

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag JK375_RS23545 Protein ID WP_004213072.1
Coordinates 178158..178601 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag JK375_RS23540 Protein ID WP_004213073.1
Coordinates 177931..178161 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JK375_RS23515 174154..175053 - 900 WP_004225022.1 nucleotide-binding protein -
JK375_RS23520 175043..175333 - 291 WP_004213078.1 hypothetical protein -
JK375_RS23525 175685..175891 + 207 WP_004213077.1 hypothetical protein -
JK375_RS23530 175881..176174 - 294 WP_004213076.1 hypothetical protein -
JK375_RS23535 176190..177323 - 1134 WP_004213075.1 DUF3800 domain-containing protein -
JK375_RS23540 177931..178161 + 231 WP_004213073.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JK375_RS23545 178158..178601 + 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
JK375_RS23550 178615..179031 - 417 Protein_190 restriction endonuclease -
JK375_RS23555 179035..179328 - 294 Protein_191 transposase -
JK375_RS23560 179745..180380 + 636 WP_165475474.1 response regulator -
JK375_RS23565 180895..181301 - 407 Protein_193 GAF domain-containing protein -
JK375_RS23570 181392..182312 - 921 WP_004213934.1 DUF1471 domain-containing protein -
JK375_RS23575 182361..182852 - 492 WP_011251327.1 DM13 domain-containing protein -
JK375_RS23580 182915..183190 - 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(7'')-Ia rmpA / iutA / iucD / iucC / iucB / iucA / iroN / iroD / iroC / iroB 1..324396 324396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T187653 WP_004213072.1 NZ_CP068707:178158-178601 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T187653 NZ_CP089934:c2536287-2536198 [Erwinia tracheiphila]
AAGCCTGCATTAATGCCAACTTTTAGCGCATGGCTCTGAACAGAGCCATTTCCCTGGACTGAAGACAGGAGTTGTCTTCA
GTCTTTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT187653 WP_004213073.1 NZ_CP068707:177931-178161 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT187653 NZ_CP089934:2536197-2536287 [Erwinia tracheiphila]
TAAAAAAAGACTGAAGACAACTCCTGTCTTCAGTCCAGGGAAATGGCTCTGTTCAGAGCCATGCGCTAAAAGTTGGCATT
AATGCAGGCTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q6U619

Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References