Detailed information of TA system TA187652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 133308..134059
• Replicon: plasmid p1-pV-hybrid-1
• Accession: NZ_CP068707
• Organism: Escherichia coli strain J53-p1-pV-hybrid-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: JK375_RS23310
• Protein ID: WP_048333033.1
• Coordinates: 133577..134059 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: JK375_RS23305
• Protein ID: WP_004902250.1
• Coordinates: 133308..133586 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK375_RS23285	130579..131061	+	483	WP_172694439.1	hypothetical protein	-
JK375_RS23290	131305..131619	-	315	WP_023288299.1	hypothetical protein	-
JK375_RS23295	132525..132866	+	342	WP_032437747.1	hypothetical protein	-
JK375_RS23300	132974..133186	+	213	WP_016946198.1	hypothetical protein	-
JK375_RS23305	133308..133586	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
JK375_RS23310	133577..134059	+	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
JK375_RS23315	135094..135633	+	540	WP_004902239.1	hypothetical protein	-
JK375_RS23320	135738..136130	+	393	WP_032442757.1	hypothetical protein	-
JK375_RS23325	136231..136986	+	756	WP_004902235.1	DUF2971 domain-containing protein	-
JK375_RS23330	137013..137660	+	648	WP_014386537.1	EcsC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(7'')-Ia	rmpA / iutA / iucD / iucC / iucB / iucA / iroN / iroD / iroC / iroB	1..324396	324396

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T187652 WP_048333033.1 NZ_CP068707:133577-134059 [Escherichia coli]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T187652 NZ_CP089934:1408122-1408406 [Erwinia tracheiphila]
GTGTATAAACTCACCGAACGGGCGGCAGAAGACTTCGCCGGGATTTATGACTATACGCTATTAAAATTTGGTGAAGCTCA
GGCAGACCATTATACAGACGCACTTGAGGCATTTTTCGAAACACTGTCCGGAATGCCTGATATGGGGCGGGACTACAATG
CCGTCCCTGGTGTGATGCGCATTGAATTTCAGCGACATATCATTTTTTATACGGTTCGTGATACAGATATTCTGATTGCG
CGTATTTTGCATCAACAGATGAACCATAAACGCCATTTACGTTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT187652 WP_004902250.1 NZ_CP068707:133308-133586 [Escherichia coli]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT187652 NZ_CP089934:1407887-1408129 [Erwinia tracheiphila]
ATGGCCAGAACAACAAGCGTTACGATTGGCGAACAGCTCGATTCGTTTATCAGTCGATTAATAGACAGTGGCCGTTACGG
CTCTGCGAGTGAAGTTATGCGCTCTGCTCTGCGGTTGCTGGAGCAACAGGAAACAAACGATGAAGTTGTTCGCCAGGCTG
TTATTGCTGGGCTGGAAAGCGGTGAAAGCTCGCTATCACTGCGGGATATTGCAGCACAAAGAAAACTGAGACATCGTGTA
TAA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3