Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 104498..105020 Replicon plasmid p1-pV-hybrid-1
Accession NZ_CP068707
Organism Escherichia coli strain J53-p1-pV-hybrid-1

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag JK375_RS23150 Protein ID WP_004181778.1
Coordinates 104498..104782 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag JK375_RS23155 Protein ID WP_004181777.1
Coordinates 104772..105020 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JK375_RS23120 99699..100794 + 1096 Protein_104 conjugal transfer protein TraG N-terminal domain-containing protein -
JK375_RS23125 100893..102080 - 1188 WP_131860749.1 transposase -
JK375_RS23130 102116..102544 + 429 WP_077257669.1 IS200/IS605 family transposase -
JK375_RS23135 102623..102847 + 225 Protein_107 transposase -
JK375_RS23140 103118..104344 - 1227 WP_040209639.1 transposase -
JK375_RS23145 104380..104481 + 102 Protein_109 IS200/IS605 family transposase -
JK375_RS23150 104498..104782 - 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JK375_RS23155 104772..105020 - 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JK375_RS23160 105311..107110 + 1800 WP_004181776.1 ATP-dependent helicase -
JK375_RS23165 107444..108430 + 987 WP_025368599.1 hypothetical protein -
JK375_RS23170 108575..108928 + 354 WP_004181774.1 hypothetical protein -
JK375_RS23175 108990..109811 + 822 WP_004181772.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(7'')-Ia rmpA / iutA / iucD / iucC / iucB / iucA / iroN / iroD / iroC / iroB 1..324396 324396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T187651 WP_004181778.1 NZ_CP068707:c104782-104498 [Escherichia coli]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T187651 NZ_CP089934:1130459-1130860 [Erwinia tracheiphila]
GTGGCCCTGTGGCACTCTGAGCTGCGGGTCTCATGGCGAGCACAGTGGTTTTCATTGCTGCTGCACGGCGTGGCGATCCT
GATTGTGCTGCTGGCACCCTGGCCGGCCAGCTATACGGTGGTGTGGATGTCACTCCTCACGCTAACGATATTTGAGTGCG
TGCGGAGCCAGCGGAGAATAGGCAACCGTGAGGGAGAAATTGCCCTGTTTGAGGGGCGGCAGGTGCAATGGCGGCAGAAG
CGCTGGCAGATTCGCGGGCGACCCTGGATTACCCGACAGGCTATTTTAATGACGCTGCGTTCAGCAACCGGAGAACGCGA
AAAGCTTTGGTTATTTTCAGACAGTATGTCCGCGGAAGACTGGCGATCGCTTCGAAAGCAATTACTTCGGGCGGCTCTAT
AG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT187651 WP_004181777.1 NZ_CP068707:c105020-104772 [Escherichia coli]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT187651 NZ_CP089934:1130212-1130478 [Erwinia tracheiphila]
ATGGATATAAGTAATAAAGCCCGTGTTCACTGGGCCTGCCGTCGTGGCATGCGTGAACTGGATATCTCTATTATGCCGTT
TTTTGAATATGAGTACGATTCACTGAGCGACAGTGACAAACTGGTTTTTGTACGCCTGTTGGAAAATGACGATCCCGATC
TTTTTAACTGGCTGATGAATTACGGCGAGCCGCCAGACACCGAGCTGAAACGTATGGTGCTTCTCATTCAGCAGCGAAAC
AAGCAACGTGGCCCTGTGGCACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References