Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3354027..3354645 Replicon chromosome
Accession NZ_CP068706
Organism Escherichia coli strain J53-p1-pV-hybrid-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JK375_RS16410 Protein ID WP_001291435.1
Coordinates 3354427..3354645 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JK375_RS16405 Protein ID WP_000344800.1
Coordinates 3354027..3354401 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JK375_RS16395 3349116..3350309 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JK375_RS16400 3350332..3353481 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
JK375_RS16405 3354027..3354401 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JK375_RS16410 3354427..3354645 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JK375_RS16415 3354817..3355368 + 552 WP_000102564.1 maltose O-acetyltransferase -
JK375_RS16420 3355484..3355954 + 471 WP_000136192.1 YlaC family protein -
JK375_RS16425 3356118..3357668 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JK375_RS16430 3357710..3358063 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JK375_RS16440 3358442..3358753 + 312 WP_000409911.1 MGMT family protein -
JK375_RS16445 3358784..3359356 - 573 WP_000779842.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T187643 WP_001291435.1 NZ_CP068706:3354427-3354645 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T187643 NZ_CP089932:c3686775-3686392 [Erwinia tracheiphila]
ATGAAATGGATTAGCGCTCAGGAGGTGATAGCGTTTCATGACCGTCTGCTTACAGCTTTGCCGGGCGTTCAGGGAATGCC
AGAGCCGGGAAGAGCAGAGGCGATCATTTATCGTGTTCAGAACCAGCTTTACTATGAAGGTGTTGAGGATATTCACGAAC
TGGCTGCAATGTATCTTGTCGCTATTTCGCGTGGTCATATCTTCAATGACGGCAATAAGCGAACCGCCTTTTTCGTTGCG
ATGGCTTTTTTAAAGCGCAATGGCATTGATATCCGTGACGAAGGTGATGAACTGGAAGAACTGACGGTATCCGCAGCGAT
GGGGGCAGTAAGCAGCGATGATATTGCTGCTGTTTTGCGCCGATTATCCATCAATCCCCACTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT187643 WP_000344800.1 NZ_CP068706:3354027-3354401 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT187643 NZ_CP089932:c3686993-3686772 [Erwinia tracheiphila]
ATGCGAACCTATACCACATCTCAGGCCCGGCAAAATATTGCCGAAGTAATGGAAGCGGCTACGGCTGGTGAGCCTGTAGA
AATAACTCGCCGCGATGGCTCTGCCGCTGTGCTTATCAGCCGAGATGATTTCAACGCGTGGCAGGAAGCAAAGCTGGATG
CTGAATTTGCCGAAATTATGGGGCGACACGGTCGTACCATTAAGGCGCTGGCCGACAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References