Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2274944..2275582 Replicon chromosome
Accession NZ_CP068706
Organism Escherichia coli strain J53-p1-pV-hybrid-1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JK375_RS11035 Protein ID WP_000813794.1
Coordinates 2275406..2275582 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JK375_RS11030 Protein ID WP_001270286.1
Coordinates 2274944..2275360 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JK375_RS11010 2270096..2271037 - 942 WP_001251304.1 ABC transporter permease -
JK375_RS11015 2271038..2272051 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
JK375_RS11020 2272069..2273214 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
JK375_RS11025 2273459..2274865 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
JK375_RS11030 2274944..2275360 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JK375_RS11035 2275406..2275582 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JK375_RS11040 2275804..2276034 + 231 WP_000494244.1 YncJ family protein -
JK375_RS11045 2276126..2278087 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JK375_RS11050 2278160..2278696 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
JK375_RS11055 2278749..2279963 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T187642 WP_000813794.1 NZ_CP068706:c2275582-2275406 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T187642 NZ_CP089932:c3613001-3612600 [Erwinia tracheiphila]
GTGGCCCTGTGGCACTCTGAGCTGCGGGTCTCATGGCGAGCACAGTGGTTTTCATTGCTGCTGCACGGCGTGGCGATCCT
GATTGTGCTGCTGGCACCCTGGCCGGCCAGCTATACGGTGGTGTGGATGTCACTCCTCACGCTAACGATATTTGAGTGCG
TGCGGAGCCAGCGGAGAATAGGCAACCGTGAGGGAGAAATTGCCCTGTTTGAGGGGCGGCAGGTGCAATGGCGGCAGAAG
CGCTGGCAGATTCGCGGGCGACCCTGGATTACCCGACAGGCTATTTTAATGACGCTGCGTTCAGCAACCGGAGAACGCGA
AAAGCTTTGGTTATTTTCAGACAGTATGTCCGCGGAAGACTGGCGATCGCTTCGAAAGCAATTACTTCGGGCGGCTCTAT
AG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT187642 WP_001270286.1 NZ_CP068706:c2275360-2274944 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT187642 NZ_CP089932:c3613248-3612982 [Erwinia tracheiphila]
ATGGATATAAGTAATAAAGCCCGTGTTCACTGGGCCTGCCGTCGTGGCATGCGTGAACTGGATATCTCTATTATGCCGTT
TTTTGAATATGAGTACGATTCACTGAGCGACAGTGACAAACTGGTTTTTGTACGCCTGTTGGAAAATGACGATCCCGATC
TTTTTAACTGGCTGATGAATTACGGCGAGCCGCCAGACACCGAGCTGAAACGTATGGTGCTTCTCATTCAGCAGCGAAAC
AAGCAACGTGGCCCTGTGGCACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References