Detailed information of TA system TA187642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2274944..2275582
• Replicon: chromosome
• Accession: NZ_CP068706
• Organism: Escherichia coli strain J53-p1-pV-hybrid-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JK375_RS11035
• Protein ID: WP_000813794.1
• Coordinates: 2275406..2275582 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JK375_RS11030
• Protein ID: WP_001270286.1
• Coordinates: 2274944..2275360 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK375_RS11010	2270096..2271037	-	942	WP_001251304.1	ABC transporter permease	-
JK375_RS11015	2271038..2272051	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
JK375_RS11020	2272069..2273214	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
JK375_RS11025	2273459..2274865	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
JK375_RS11030	2274944..2275360	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JK375_RS11035	2275406..2275582	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JK375_RS11040	2275804..2276034	+	231	WP_000494244.1	YncJ family protein	-
JK375_RS11045	2276126..2278087	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JK375_RS11050	2278160..2278696	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
JK375_RS11055	2278749..2279963	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T187642 WP_000813794.1 NZ_CP068706:c2275582-2275406 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T187642 NZ_CP089932:c3613001-3612600 [Erwinia tracheiphila]
GTGGCCCTGTGGCACTCTGAGCTGCGGGTCTCATGGCGAGCACAGTGGTTTTCATTGCTGCTGCACGGCGTGGCGATCCT
GATTGTGCTGCTGGCACCCTGGCCGGCCAGCTATACGGTGGTGTGGATGTCACTCCTCACGCTAACGATATTTGAGTGCG
TGCGGAGCCAGCGGAGAATAGGCAACCGTGAGGGAGAAATTGCCCTGTTTGAGGGGCGGCAGGTGCAATGGCGGCAGAAG
CGCTGGCAGATTCGCGGGCGACCCTGGATTACCCGACAGGCTATTTTAATGACGCTGCGTTCAGCAACCGGAGAACGCGA
AAAGCTTTGGTTATTTTCAGACAGTATGTCCGCGGAAGACTGGCGATCGCTTCGAAAGCAATTACTTCGGGCGGCTCTAT
AG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT187642 WP_001270286.1 NZ_CP068706:c2275360-2274944 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT187642 NZ_CP089932:c3613248-3612982 [Erwinia tracheiphila]
ATGGATATAAGTAATAAAGCCCGTGTTCACTGGGCCTGCCGTCGTGGCATGCGTGAACTGGATATCTCTATTATGCCGTT
TTTTGAATATGAGTACGATTCACTGAGCGACAGTGACAAACTGGTTTTTGTACGCCTGTTGGAAAATGACGATCCCGATC
TTTTTAACTGGCTGATGAATTACGGCGAGCCGCCAGACACCGAGCTGAAACGTATGGTGCTTCTCATTCAGCAGCGAAAC
AAGCAACGTGGCCCTGTGGCACTCTGA

Structures

Toxin

• PDB: 6HPB