Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2138996..2139522 Replicon chromosome
Accession NZ_CP068706
Organism Escherichia coli strain J53-p1-pV-hybrid-1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag JK375_RS10360 Protein ID WP_000323025.1
Coordinates 2139235..2139522 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag JK375_RS10355 Protein ID WP_000534858.1
Coordinates 2138996..2139235 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JK375_RS10300 2134023..2134790 - 768 Protein_2012 exonuclease -
JK375_RS10305 2134883..2135074 - 192 WP_001083297.1 lysis protein YdfD -
JK375_RS10310 2135071..2135259 - 189 WP_000854559.1 cell division inhibition protein DicB -
JK375_RS10315 2135827..2136045 - 219 WP_001171942.1 hypothetical protein -
JK375_RS10320 2136205..2136360 - 156 WP_000379575.1 DUF1391 family protein -
JK375_RS10325 2136527..2136934 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
JK375_RS10330 2137018..2137248 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
JK375_RS10335 2137232..2137510 + 279 Protein_2019 hypothetical protein -
JK375_RS10340 2137545..2137694 + 150 WP_011443592.1 hypothetical protein -
JK375_RS10345 2138131..2138463 - 333 WP_001301033.1 protein FlxA -
JK375_RS10350 2138666..2138971 - 306 WP_001326990.1 hypothetical protein -
JK375_RS10355 2138996..2139235 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
JK375_RS10360 2139235..2139522 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
JK375_RS10365 2139594..2139749 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
JK375_RS10370 2139966..2140217 + 252 WP_000980994.1 hypothetical protein -
JK375_RS10375 2140284..2140562 + 279 WP_012304870.1 hypothetical protein -
JK375_RS10380 2140564..2141613 + 1050 WP_001265198.1 DUF968 domain-containing protein -
JK375_RS10385 2141627..2142379 + 753 WP_001047135.1 antitermination protein -
JK375_RS10390 2142657..2142746 - 90 WP_120795389.1 hypothetical protein -
JK375_RS10395 2142801..2143013 - 213 WP_000087756.1 cold shock-like protein CspF -
JK375_RS10400 2143314..2143529 + 216 WP_000066484.1 cold shock-like protein CspB -
JK375_RS10405 2144283..2144498 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2127375..2160763 33388


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T187640 WP_000323025.1 NZ_CP068706:2139235-2139522 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T187640 NZ_CP089932:2185591-2185680 [Erwinia tracheiphila]
AAGCCTGCATTAATGCCAACTTTTAGCGCATGGCTCTGAACAGAGCCATTTCCCTGGACTGAAGACAGGAGTTGTCTTCA
GTCTTTTTTT

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT187640 WP_000534858.1 NZ_CP068706:2138996-2139235 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT187640 NZ_CP089932:c2185681-2185591 [Erwinia tracheiphila]
TAAAAAAAGACTGAAGACAACTCCTGTCTTCAGTCCAGGGAAATGGCTCTGTTCAGAGCCATGCGCTAAAAGTTGGCATT
AATGCAGGCTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References