187627

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 871683..872266 Replicon chromosome
Accession NZ_CP068706
Organism Escherichia coli strain J53-p1-pV-hybrid-1

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag JK375_RS04225 Protein ID WP_000254738.1
Coordinates 871931..872266 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag JK375_RS04220 Protein ID WP_000581937.1
Coordinates 871683..871931 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JK375_RS04210 868022..869323 + 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
JK375_RS04215 869371..871605 + 2235 WP_000226815.1 GTP pyrophosphokinase -
JK375_RS04220 871683..871931 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JK375_RS04225 871931..872266 + 336 WP_000254738.1 endoribonuclease MazF Toxin
JK375_RS04230 872337..873128 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
JK375_RS04235 873356..874993 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JK375_RS04240 875081..876379 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T187627 WP_000254738.1 NZ_CP068706:871931-872266 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T187627 NZ_CP089927:4045807-4046208 [Mycobacterium tuberculosis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT187627 WP_000581937.1 NZ_CP068706:871683-871931 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT187627 NZ_CP089927:4045583-4045810 [Mycobacterium tuberculosis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References