Detailed information of TA system TA187596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 1779..2359
• Replicon: plasmid pRJBSI76-4
• Accession: NZ_CP068693
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: I5002_RS30575
• Protein ID: WP_071177730.1
• Coordinates: 2045..2359 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: I5002_RS30570
• Protein ID: WP_000093040.1
• Coordinates: 1779..2057 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5002_RS30555	352..597	+	246	WP_032440458.1	hypothetical protein	-
I5002_RS30560	871..1242	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
I5002_RS30565	1239..1445	+	207	Protein_2	TonB family protein	-
I5002_RS30570	1779..2057	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
I5002_RS30575	2045..2359	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5002_RS30580	2523..2951	+	429	WP_001140599.1	hypothetical protein	-
I5002_RS30585	2977..3156	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
I5002_RS30590	3183..3713	-	531	WP_071177729.1	hypothetical protein	-
I5002_RS30595	3720..4451	-	732	WP_071177728.1	replication-relaxation family protein	-
I5002_RS30600	4451..6415	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10206	10206

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T187596 WP_071177730.1 NZ_CP068693:2045-2359 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T187596 NZ_CP089927:c1285998-1285564 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT187596 WP_000093040.1 NZ_CP068693:1779-2057 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT187596 NZ_CP089927:c1286259-1286005 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1