Detailed information of TA system TA187586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 117369..118105
• Replicon: plasmid pRJBSI76-2
• Accession: NZ_CP068691
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: I5002_RS28845
• Protein ID: WP_003026803.1
• Coordinates: 117623..118105 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I5002_RS28840
• Protein ID: WP_003026799.1
• Coordinates: 117369..117635 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5002_RS28795	113431..113793	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
I5002_RS28800	113843..114193	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
I5002_RS28805	114551..114820	+	270	WP_004152102.1	hypothetical protein	-
I5002_RS28810	114808..115383	+	576	WP_004152103.1	hypothetical protein	-
I5002_RS28815	115414..115908	+	495	WP_004152104.1	DNA-binding protein	-
I5002_RS28820	115952..116320	+	369	WP_004152105.1	hypothetical protein	-
I5002_RS28825	116354..116557	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
I5002_RS28830	116606..116863	+	258	WP_004152107.1	hypothetical protein	-
I5002_RS28835	116939..117193	+	255	WP_004152108.1	hypothetical protein	-
I5002_RS28840	117369..117635	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I5002_RS28845	117623..118105	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
I5002_RS28850	118317..119663	+	1347	WP_020314316.1	ISNCY family transposase	-
I5002_RS28855	120164..121418	-	1255	Protein_123	IS3 family transposase	-
I5002_RS28860	121506..122468	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / mph(A) / sul1 / blaDHA-1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(D) / floR / blaCTX-M-27	-	1..206263	206263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T187586 WP_003026803.1 NZ_CP068691:117623-118105 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T187586 NZ_CP089927:c871483-871094 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT187586 WP_003026799.1 NZ_CP068691:117369-117635 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT187586 NZ_CP089927:c871707-871480 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66