Detailed information of TA system TA187585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 290327..291237
• Replicon: plasmid pRJBSI76-1
• Accession: NZ_CP068690
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: I5002_RS28180
• Protein ID: WP_004181896.1
• Coordinates: 290327..290797 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: I5002_RS28185
• Protein ID: WP_004181895.1
• Coordinates: 290794..291237 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5002_RS28160	286365..287333	-	969	WP_176691886.1	IS5-like element IS903B family transposase	-
I5002_RS28165	287975..288133	-	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
I5002_RS28170	288205..289386	+	1182	WP_004883380.1	IS481 family transposase	-
I5002_RS28175	289957..290217	+	261	WP_004026352.1	hypothetical protein	-
I5002_RS28180	290327..290797	-	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
I5002_RS28185	290794..291237	-	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
I5002_RS28190	291338..292609	-	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
I5002_RS28195	292609..293031	-	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
I5002_RS28200	293117..294604	-	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
I5002_RS28205	295296..295865	-	570	Protein_311	transposase	-
I5002_RS28210	295867..296082	+	216	Protein_312	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	rmpA / iutA / iucD / iucC / iucB / iucA / rmpA	1..302690	302690
	-	flank	IS/Tn	-	-	295296..295649	353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T187585 WP_004181896.1 NZ_CP068690:c290797-290327 [Klebsiella pneumoniae subsp. pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T187585 NZ_CP089927:689159-689563 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT187585 WP_004181895.1 NZ_CP068690:c291237-290794 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT187585 NZ_CP089927:688950-689153 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9