Detailed information of TA system TA187584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 178049..178719
• Replicon: plasmid pRJBSI76-1
• Accession: NZ_CP068690
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: I5002_RS27590
• Protein ID: WP_004213072.1
• Coordinates: 178276..178719 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: I5002_RS27585
• Protein ID: WP_004213073.1
• Coordinates: 178049..178279 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5002_RS27560	174272..175171	-	900	WP_004225022.1	nucleotide-binding protein	-
I5002_RS27565	175161..175451	-	291	WP_004213078.1	hypothetical protein	-
I5002_RS27570	175803..176009	+	207	WP_004213077.1	hypothetical protein	-
I5002_RS27575	175999..176292	-	294	WP_004213076.1	hypothetical protein	-
I5002_RS27580	176308..177441	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
I5002_RS27585	178049..178279	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I5002_RS27590	178276..178719	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
I5002_RS27595	178733..179149	-	417	Protein_189	restriction endonuclease	-
I5002_RS27600	179153..179446	-	294	Protein_190	transposase	-
I5002_RS27605	179863..180498	+	636	WP_165475474.1	response regulator	-
I5002_RS27610	181013..181419	-	407	Protein_192	GAF domain-containing protein	-
I5002_RS27615	181510..182430	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
I5002_RS27620	182479..182970	-	492	WP_011251327.1	DM13 domain-containing protein	-
I5002_RS27625	183033..183308	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	rmpA / iutA / iucD / iucC / iucB / iucA / rmpA	1..302690	302690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T187584 WP_004213072.1 NZ_CP068690:178276-178719 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T187584 NZ_CP089927:610750-611067 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT187584 WP_004213073.1 NZ_CP068690:178049-178279 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT187584 NZ_CP089927:610451-610753 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620