Detailed information of TA system TA187583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 133426..134177
• Replicon: plasmid pRJBSI76-1
• Accession: NZ_CP068690
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: I5002_RS27355
• Protein ID: WP_048333033.1
• Coordinates: 133695..134177 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: I5002_RS27350
• Protein ID: WP_004902250.1
• Coordinates: 133426..133704 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5002_RS27330	130697..131179	+	483	WP_172694439.1	hypothetical protein	-
I5002_RS27335	131423..131737	-	315	WP_023288299.1	hypothetical protein	-
I5002_RS27340	132643..132984	+	342	WP_032437747.1	hypothetical protein	-
I5002_RS27345	133092..133304	+	213	WP_016946198.1	hypothetical protein	-
I5002_RS27350	133426..133704	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
I5002_RS27355	133695..134177	+	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
I5002_RS27360	135212..135751	+	540	WP_004902239.1	hypothetical protein	-
I5002_RS27365	135856..136248	+	393	WP_032442757.1	hypothetical protein	-
I5002_RS27370	136349..137104	+	756	WP_004902235.1	DUF2971 domain-containing protein	-
I5002_RS27375	137131..137778	+	648	WP_014386537.1	EcsC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	rmpA / iutA / iucD / iucC / iucB / iucA / rmpA	1..302690	302690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T187583 WP_048333033.1 NZ_CP068690:133695-134177 [Klebsiella pneumoniae subsp. pneumoniae]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T187583 NZ_CP089927:c499431-499030 [Mycobacterium tuberculosis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGACCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT187583 WP_004902250.1 NZ_CP068690:133426-133704 [Klebsiella pneumoniae subsp. pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT187583 NZ_CP089927:c499685-499428 [Mycobacterium tuberculosis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3