Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5362605..5363230 Replicon chromosome
Accession NZ_CP068689
Organism Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I5002_RS26450 Protein ID WP_002882817.1
Coordinates 5362605..5362988 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I5002_RS26455 Protein ID WP_004150355.1
Coordinates 5362988..5363230 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5002_RS26435 5359971..5360873 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I5002_RS26440 5360870..5361505 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I5002_RS26445 5361502..5362431 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I5002_RS26450 5362605..5362988 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I5002_RS26455 5362988..5363230 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I5002_RS26460 5363435..5364352 + 918 WP_202852352.1 alpha/beta hydrolase -
I5002_RS26465 5364366..5365307 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I5002_RS26470 5365352..5365789 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I5002_RS26475 5365786..5366646 - 861 WP_002882807.1 virulence factor BrkB family protein -
I5002_RS26480 5366640..5367239 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T187581 WP_002882817.1 NZ_CP068689:c5362988-5362605 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T187581 NZ_CP089927:c314294-314001 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT187581 WP_004150355.1 NZ_CP068689:c5363230-5362988 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT187581 NZ_CP089927:c314560-314291 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References