Detailed information of TA system TA187576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2798441..2800029
• Replicon: chromosome
• Accession: NZ_CP068689
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0H3GN97
• Locus tag: I5002_RS13830
• Protein ID: WP_004199851.1
• Coordinates: 2798441..2799763 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: I5002_RS13835
• Protein ID: WP_002904790.1
• Coordinates: 2799763..2800029 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5002_RS13815	2793506..2796007	+	2502	WP_004199853.1	fimbrial biogenesis outer membrane usher protein	-
I5002_RS13820	2795995..2796999	+	1005	WP_004199852.1	type 1 fimbrial protein	-
I5002_RS13825	2797132..2798424	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
I5002_RS13830	2798441..2799763	-	1323	WP_004199851.1	type II toxin-antitoxin system HipA family toxin	Toxin
I5002_RS13835	2799763..2800029	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I5002_RS13840	2800295..2800621	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
I5002_RS13845	2800618..2801118	-	501	WP_002904787.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
I5002_RS13850	2801096..2802475	-	1380	WP_004199850.1	purine permease	-
I5002_RS13855	2802821..2803975	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
I5002_RS13860	2803956..2804879	-	924	WP_004199849.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49128.44 Da
• Isoelectric Point: 7.2487

>T187576 WP_004199851.1 NZ_CP068689:c2799763-2798441 [Klebsiella pneumoniae subsp. pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPLEQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T187576 NZ_CP089926:c5217390-5217007 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT187576 WP_002904790.1 NZ_CP068689:c2800029-2799763 [Klebsiella pneumoniae subsp. pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT187576 NZ_CP089926:c5217632-5217390 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATACCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0H3GN97

Antitoxin

• AlphaFold DB: A0A0H3GXD8