Detailed information of TA system TA187563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 1779..2359
• Replicon: plasmid pRJBSI76-pV-4
• Accession: NZ_CP068688
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76-pV

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: JMY88_RS30515
• Protein ID: WP_071177730.1
• Coordinates: 2045..2359 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: JMY88_RS30510
• Protein ID: WP_000093040.1
• Coordinates: 1779..2057 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMY88_RS30495	352..597	+	246	WP_032440458.1	hypothetical protein	-
JMY88_RS30500	871..1242	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
JMY88_RS30505	1239..1445	+	207	Protein_2	TonB family protein	-
JMY88_RS30510	1779..2057	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
JMY88_RS30515	2045..2359	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JMY88_RS30520	2523..2951	+	429	WP_001140599.1	hypothetical protein	-
JMY88_RS30525	2977..3156	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
JMY88_RS30530	3183..3713	-	531	WP_071177729.1	hypothetical protein	-
JMY88_RS30535	3720..4451	-	732	WP_071177728.1	replication-relaxation family protein	-
JMY88_RS30540	4451..6415	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10206	10206

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T187563 WP_071177730.1 NZ_CP068688:2045-2359 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T187563 NZ_CP089920:c384710-383385 [Rahnella victoriana]
ATGCGTCGTGCGCAGCAGCGTTTAGCAATATGGATGAACGGTATTCCGGTGGGATACTGGGATAAGCAAAAAGGTGAGGA
TCGTTTACAGTACTTGCCCTCCTGGAGGGAGGATCAACAAGGGCGACCTTTGTCGCTTTCCCTGCCGTTTACGCCGGGTA
ATCAGGTACACCGTGGCAATGTGGTTCGTGATTATTTTGATAATTTATTGCCCGACAGCGAAGGGATCCGCAGGCGTTTG
GCCGCACGTTATCATGCTGAGAGCCTCGAGCCTTTTGATTTGCTCACAGAGTTAGGGCGGGACTGCGTCGGTGCAATCCA
GTTGCTGAATGTCGGGGATCAACCTGCTGGGATATTCACTATTCGCCAGCGCCTGCTTTCAGAACCGGAGATCGCCGGTA
TGCTGCGCAACACGGCGGGGATGTTGCCGGGCCAGAACAATCAGGACGAAGATTTGCGATTGTCTATCGCCGGTGCACAG
GAAAAAACCGCGCTATTATGGCACAACGGGCAATGGTGTTTGCCGGAAGGCAATACGCCAACCACGCATATTTTTAAATT
GCCGCTGGGTCTGGTGGGAAATATGCAGGCAGATATGAGTACGTCCGTTGAAAACGAATGGATATGTTCATTGATAATTG
AGCAGTTTGGTCTGGCGGTGGCTAAAGCTCAGGTTGCGCAATTTGAGGATCAGAAAGCGCTGATCGTCGAACGTTTTGAC
CGAAAGCTTTCTTCAGACGGTGGATGGTGGATCCGTCTGCCGCAGGAAGATATGTGTCAGGCGATGGGCGTATCACCTTT
GCGGAAATATCAGTCCGATGGCGGCCCTGGGATCACGGACATCATGGAAATATTGAGCCGGTCTGAGCAGGCGCAGCAGG
ATCAGTCTCAGTTTTTCAAAGCGCAGATTATTTTCTGGTTGATGGCCGCCACTGATGGTCATGCTAAAAATTTCAGCCTC
GCGCATCTTCCGCAAAATCGTTATTGCATGACGCCGCTGTACGATATTTTGTCAGCATGGCCGGTCATCGGCCATGGAAA
CAATAAGATTGCGTGGCAAAAATGTAAGCTGGCGATGGCCGTCCGTGGCAGCAGTAATTATTACCTGATCAGCAAGATCC
AGCGCAGACATTGGATTAAGCAGGGCGAGCAGAGCGGGTTAGGTAAACGGCAAATAGAAACCATGATCGATGAGATCATT
GCGCTCGTACCGGTCGTTATTGAAAATGTTGCAAGGCAGCTCCCTGCTTCGTTTCCTCCTGAACTGGCAGACGCTATTTT
TACCGGGATGGAACAGCAACGGGTGCGGCTGCAAGCACAGGCTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT187563 WP_000093040.1 NZ_CP068688:1779-2057 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT187563 NZ_CP089920:c385011-384715 [Rahnella victoriana]
ATGAAGACAGTCTATCCATTGAAGACACTTAATCAGCTCCGTCCGTTCCTGGTGGGATTTCGTAAGGAAAAAGGTCTGAC
ACAAAAGGACGTGTCAGAAAAACTGGGCGTGACCCAGCAGACGTATGCCCGGCTGGAGGCAAACCCTGCCGCTGCCAGCC
TTGACCGGCTGTTTCGGGTATTCAATGTGTTAGGTATTGAGATGGTATTTTCTTCACAATCCGATCCTGAAAAGCCTTCT
ATGGAGGAAATTAAGCAGGATTATTTAAGTACGCCCGCCAGAAAGGAACAATGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1