Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 34651..35294 Replicon plasmid pRJBSI76-pV-3
Accession NZ_CP068687
Organism Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76-pV

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag JMY88_RS29510 Protein ID WP_001044770.1
Coordinates 34651..35067 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag JMY88_RS29515 Protein ID WP_001261282.1
Coordinates 35064..35294 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMY88_RS29490 30754..31026 - 273 Protein_47 transposase -
JMY88_RS29495 31055..31752 - 698 Protein_48 IS1 family transposase -
JMY88_RS29500 32008..33030 - 1023 WP_000361404.1 helicase UvrD -
JMY88_RS29505 33015..34577 - 1563 WP_004206609.1 AAA family ATPase -
JMY88_RS29510 34651..35067 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
JMY88_RS29515 35064..35294 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JMY88_RS29520 35251..35712 + 462 WP_014343465.1 hypothetical protein -
JMY88_RS29525 35873..36817 + 945 WP_011977810.1 hypothetical protein -
JMY88_RS29530 36854..37246 + 393 WP_011977811.1 hypothetical protein -
JMY88_RS29535 37304..37825 + 522 WP_013214008.1 hypothetical protein -
JMY88_RS29540 37871..38074 + 204 WP_011977813.1 hypothetical protein -
JMY88_RS29545 38104..39108 + 1005 WP_011977814.1 hypothetical protein -
JMY88_RS29550 39292..40071 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / rmtB / blaTEM-1B - 1..184751 184751


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T187554 WP_001044770.1 NZ_CP068687:c35067-34651 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T187554 NZ_CP089886:31684-31989 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT187554 WP_001261282.1 NZ_CP068687:c35294-35064 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT187554 NZ_CP089886:31464-31682 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References