Detailed information of TA system TA187553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 212912..213582
• Replicon: plasmid pRJBSI76-pV-2
• Accession: NZ_CP068686
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76-pV

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: JMY88_RS29180
• Protein ID: WP_004213072.1
• Coordinates: 212912..213355 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: JMY88_RS29185
• Protein ID: WP_004213073.1
• Coordinates: 213352..213582 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMY88_RS29145	208321..208596	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
JMY88_RS29150	208659..209150	+	492	WP_011251327.1	DM13 domain-containing protein	-
JMY88_RS29155	209199..210119	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
JMY88_RS29160	210210..210616	+	407	Protein_222	GAF domain-containing protein	-
JMY88_RS29165	211132..211768	-	637	Protein_223	mucoid phenotype A regulator RmpA	-
JMY88_RS29170	212185..212478	+	294	Protein_224	transposase	-
JMY88_RS29175	212482..212898	+	417	Protein_225	restriction endonuclease	-
JMY88_RS29180	212912..213355	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
JMY88_RS29185	213352..213582	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JMY88_RS29190	214190..215323	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
JMY88_RS29195	215339..215632	+	294	WP_004213076.1	hypothetical protein	-
JMY88_RS29200	215622..215828	-	207	WP_004213077.1	hypothetical protein	-
JMY88_RS29205	216180..216470	+	291	WP_004213078.1	hypothetical protein	-
JMY88_RS29210	216460..217359	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aph(7'')-Ia	iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2	1..225445	225445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T187553 WP_004213072.1 NZ_CP068686:c213355-212912 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T187553 NZ_CP089885:229246-229728 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT187553 WP_004213073.1 NZ_CP068686:c213582-213352 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT187553 NZ_CP089885:228992-229258 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620