Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 254135..255045 Replicon plasmid pRJBSI76-pV-1
Accession NZ_CP068685
Organism Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76-pV

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag JMY88_RS28005 Protein ID WP_004181896.1
Coordinates 254135..254605 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag JMY88_RS28010 Protein ID WP_004181895.1
Coordinates 254602..255045 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMY88_RS27985 250173..251141 - 969 WP_176691886.1 IS5-like element IS903B family transposase -
JMY88_RS27990 251783..251941 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
JMY88_RS27995 252013..253194 + 1182 WP_004883380.1 IS481 family transposase -
JMY88_RS28000 253765..254025 + 261 WP_004026352.1 hypothetical protein -
JMY88_RS28005 254135..254605 - 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
JMY88_RS28010 254602..255045 - 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
JMY88_RS28015 255146..256417 - 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
JMY88_RS28020 256417..256839 - 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
JMY88_RS28025 256925..258412 - 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
JMY88_RS28030 259104..259673 - 570 Protein_273 transposase -
JMY88_RS28035 259675..259890 + 216 Protein_274 IS200/IS605 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA 1..263776 263776
- flank IS/Tn - - 259104..259490 386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T187550 WP_004181896.1 NZ_CP068685:c254605-254135 [Klebsiella pneumoniae subsp. pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T187550 NZ_CP089884:c4772074-4771790 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCAGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT187550 WP_004181895.1 NZ_CP068685:c255045-254602 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT187550 NZ_CP089884:c4772306-4772064 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References