Detailed information of TA system TA187548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 178278..178948
• Replicon: plasmid pRJBSI76-pV-1
• Accession: NZ_CP068685
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76-pV

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: JMY88_RS27610
• Protein ID: WP_004213072.1
• Coordinates: 178505..178948 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: JMY88_RS27605
• Protein ID: WP_004213073.1
• Coordinates: 178278..178508 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMY88_RS27580	174501..175400	-	900	WP_004225022.1	nucleotide-binding protein	-
JMY88_RS27585	175390..175680	-	291	WP_004213078.1	hypothetical protein	-
JMY88_RS27590	176032..176238	+	207	WP_004213077.1	hypothetical protein	-
JMY88_RS27595	176228..176521	-	294	WP_004213076.1	hypothetical protein	-
JMY88_RS27600	176537..177670	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
JMY88_RS27605	178278..178508	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JMY88_RS27610	178505..178948	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
JMY88_RS27615	178962..179378	-	417	Protein_190	restriction endonuclease	-
JMY88_RS27620	179382..179675	-	294	Protein_191	transposase	-
JMY88_RS27625	180092..180728	+	637	Protein_192	mucoid phenotype A regulator RmpA	-
JMY88_RS27630	181244..181650	-	407	Protein_193	GAF domain-containing protein	-
JMY88_RS27635	181741..182661	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
JMY88_RS27640	182710..183201	-	492	WP_011251327.1	DM13 domain-containing protein	-
JMY88_RS27645	183264..183539	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA	1..263776	263776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T187548 WP_004213072.1 NZ_CP068685:178505-178948 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T187548 NZ_CP089884:4048867-4049085 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT187548 WP_004213073.1 NZ_CP068685:178278-178508 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT187548 NZ_CP089884:4048466-4048840 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620