Detailed information of TA system TA187547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 133655..134406
• Replicon: plasmid pRJBSI76-pV-1
• Accession: NZ_CP068685
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76-pV

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: JMY88_RS27375
• Protein ID: WP_048333033.1
• Coordinates: 133924..134406 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: JMY88_RS27370
• Protein ID: WP_004902250.1
• Coordinates: 133655..133933 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMY88_RS27350	130926..131408	+	483	WP_172694439.1	hypothetical protein	-
JMY88_RS27355	131652..131966	-	315	WP_023288299.1	hypothetical protein	-
JMY88_RS27360	132872..133213	+	342	WP_032437747.1	hypothetical protein	-
JMY88_RS27365	133321..133533	+	213	WP_016946198.1	hypothetical protein	-
JMY88_RS27370	133655..133933	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
JMY88_RS27375	133924..134406	+	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
JMY88_RS27380	135441..135980	+	540	WP_004902239.1	hypothetical protein	-
JMY88_RS27385	136085..136477	+	393	WP_032442757.1	hypothetical protein	-
JMY88_RS27390	136578..137333	+	756	WP_004902235.1	DUF2971 domain-containing protein	-
JMY88_RS27395	137360..138007	+	648	WP_014386537.1	EcsC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA	1..263776	263776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T187547 WP_048333033.1 NZ_CP068685:133924-134406 [Klebsiella pneumoniae subsp. pneumoniae]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T187547 NZ_CP089884:c3871667-3871350 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT187547 WP_004902250.1 NZ_CP068685:133655-133933 [Klebsiella pneumoniae subsp. pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT187547 NZ_CP089884:c3871357-3871070 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3