Detailed information of TA system TA187542

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4491117..4491771
• Replicon: chromosome
• Accession: NZ_CP068684
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76-pV

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R4YHX2
• Locus tag: JMY88_RS22060
• Protein ID: WP_004144731.1
• Coordinates: 4491117..4491524 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: JMY88_RS22065
• Protein ID: WP_002916312.1
• Coordinates: 4491505..4491771 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMY88_RS22040	4487117..4488850	-	1734	WP_004151783.1	single-stranded-DNA-specific exonuclease RecJ	-
JMY88_RS22045	4488856..4489569	-	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
JMY88_RS22050	4489592..4490488	-	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
JMY88_RS22055	4490589..4491110	+	522	WP_002916308.1	flavodoxin FldB	-
JMY88_RS22060	4491117..4491524	-	408	WP_004144731.1	protein YgfX	Toxin
JMY88_RS22065	4491505..4491771	-	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
JMY88_RS22070	4492017..4493000	+	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
JMY88_RS22075	4493151..4493810	-	660	WP_002916317.1	hemolysin III family protein	-
JMY88_RS22080	4493974..4494285	-	312	WP_002916319.1	N(4)-acetylcytidine aminohydrolase	-
JMY88_RS22085	4494335..4495063	+	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
JMY88_RS22090	4495182..4496615	+	1434	WP_002916322.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15936.69 Da
• Isoelectric Point: 11.4778

>T187542 WP_004144731.1 NZ_CP068684:c4491524-4491117 [Klebsiella pneumoniae subsp. pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNAE
WEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Nucleotide

• Length: 408 bp

>T187542 NZ_CP089883:c175344-174874 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT187542 WP_002916312.1 NZ_CP068684:c4491771-4491505 [Klebsiella pneumoniae subsp. pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT187542 NZ_CP089883:c175784-175341 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A378E4P6

Antitoxin

• AlphaFold DB: A0A0H3GY41