Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 42956..43581 Replicon chromosome
Accession NZ_CP068684
Organism Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76-pV

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JMY88_RS00200 Protein ID WP_002882817.1
Coordinates 43198..43581 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JMY88_RS00195 Protein ID WP_004150355.1
Coordinates 42956..43198 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMY88_RS00170 38947..39546 + 600 WP_004151865.1 glucose-1-phosphatase -
JMY88_RS00175 39540..40400 + 861 WP_002882807.1 virulence factor BrkB family protein -
JMY88_RS00180 40397..40834 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JMY88_RS00185 40879..41820 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JMY88_RS00190 41834..42751 - 918 WP_202852352.1 alpha/beta hydrolase -
JMY88_RS00195 42956..43198 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JMY88_RS00200 43198..43581 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JMY88_RS00205 43755..44684 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JMY88_RS00210 44681..45316 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JMY88_RS00215 45313..46215 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T187528 WP_002882817.1 NZ_CP068684:43198-43581 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T187528 NZ_CP089881:176929-177240 [Klebsiella pneumoniae]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT187528 WP_004150355.1 NZ_CP068684:42956-43198 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT187528 NZ_CP089881:177237-177656 [Klebsiella pneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References