Detailed information of TA system TA187525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1134863..1135392
• Replicon: chromosome
• Accession: NZ_CP068682
• Organism: Staphylococcus aureus strain NCCP11854

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JKV48_RS05550
• Protein ID: WP_000621175.1
• Coordinates: 1135030..1135392 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JKV48_RS05545
• Protein ID: WP_000948331.1
• Coordinates: 1134863..1135033 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKV48_RS05515 (JKV48_05480)	1129899..1130459	+	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-
JKV48_RS05520 (JKV48_05485)	1130668..1131147	+	480	WP_001287083.1	hypothetical protein	-
JKV48_RS05525 (JKV48_05490)	1131140..1132723	+	1584	WP_001294642.1	PH domain-containing protein	-
JKV48_RS05530 (JKV48_05495)	1132710..1133201	+	492	WP_001205915.1	PH domain-containing protein	-
JKV48_RS05535 (JKV48_05500)	1133205..1133564	+	360	WP_000581194.1	holo-ACP synthase	-
JKV48_RS05540 (JKV48_05505)	1133630..1134778	+	1149	WP_001280702.1	alanine racemase	-
JKV48_RS05545 (JKV48_05510)	1134863..1135033	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JKV48_RS05550 (JKV48_05515)	1135030..1135392	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JKV48_RS05555 (JKV48_05520)	1135742..1136743	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JKV48_RS05560 (JKV48_05525)	1136862..1137188	+	327	WP_001052491.1	anti-sigma factor antagonist	-
JKV48_RS05565 (JKV48_05530)	1137190..1137669	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
JKV48_RS05570 (JKV48_05535)	1137644..1138414	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T187525 WP_000621175.1 NZ_CP068682:1135030-1135392 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T187525 NZ_CP089880:c4585088-4584804 [Klebsiella pneumoniae]
ATGACCTATGAACTGGCGTTTGATCCACGAGCCTGGCGCGAATGGCAAAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT187525 WP_000948331.1 NZ_CP068682:1134863-1135033 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT187525 NZ_CP089880:c4585320-4585078 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1