Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4380778..4381396 Replicon chromosome
Accession NZ_CP068680
Organism Escherichia coli strain NCCP12480

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JKV47_RS21225 Protein ID WP_001291435.1
Coordinates 4381178..4381396 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JKV47_RS21220 Protein ID WP_000344800.1
Coordinates 4380778..4381152 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKV47_RS21210 (4375867) 4375867..4377060 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JKV47_RS21215 (4377083) 4377083..4380232 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JKV47_RS21220 (4380778) 4380778..4381152 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JKV47_RS21225 (4381178) 4381178..4381396 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JKV47_RS21230 (4381568) 4381568..4382119 + 552 WP_000102564.1 maltose O-acetyltransferase -
JKV47_RS21235 (4382235) 4382235..4382705 + 471 WP_000136192.1 YlaC family protein -
JKV47_RS21240 (4382869) 4382869..4384419 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JKV47_RS21245 (4384461) 4384461..4384814 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JKV47_RS21255 (4385193) 4385193..4385504 + 312 WP_000409911.1 MGMT family protein -
JKV47_RS21260 (4385535) 4385535..4386107 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T187510 WP_001291435.1 NZ_CP068680:4381178-4381396 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T187510 NZ_CP089877:c4711375-4711091 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT187510 WP_000344800.1 NZ_CP068680:4380778-4381152 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT187510 NZ_CP089877:c4711607-4711365 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References