Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3360923..3361449 Replicon chromosome
Accession NZ_CP068680
Organism Escherichia coli strain NCCP12480

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag JKV47_RS16205 Protein ID WP_000323025.1
Coordinates 3361162..3361449 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag JKV47_RS16200 Protein ID WP_000534858.1
Coordinates 3360923..3361162 (+) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKV47_RS16170 (3356285) 3356285..3356774 + 490 Protein_3143 class I SAM-dependent methyltransferase -
JKV47_RS16175 (3357252) 3357252..3357673 + 422 Protein_3144 hypothetical protein -
JKV47_RS16180 (3358329) 3358329..3359351 + 1023 Protein_3145 ISNCY family transposase -
JKV47_RS16185 (3359472) 3359472..3359621 + 150 WP_011443592.1 protein YdfW -
JKV47_RS16190 (3360058) 3360058..3360390 - 333 WP_001301033.1 FlxA-like family protein -
JKV47_RS16195 (3360593) 3360593..3360898 - 306 WP_001326990.1 protein YdfV -
JKV47_RS16200 (3360923) 3360923..3361162 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
JKV47_RS16205 (3361162) 3361162..3361449 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
JKV47_RS16210 (3361521) 3361521..3361676 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
JKV47_RS16215 (3361860) 3361860..3362120 + 261 Protein_3152 antitermination protein -
JKV47_RS16220 (3362398) 3362398..3362487 - 90 WP_120795389.1 hypothetical protein -
JKV47_RS16225 (3362542) 3362542..3362754 - 213 WP_000087756.1 cold shock-like protein CspF -
JKV47_RS16230 (3363055) 3363055..3363270 + 216 WP_000066484.1 cold shock-like protein CspB -
JKV47_RS16235 (3363634) 3363634..3363804 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
JKV47_RS16240 (3364024) 3364024..3364239 + 216 WP_000839590.1 phage lysis protein EssD -
JKV47_RS16245 (3364244) 3364244..3364555 + 312 WP_000189910.1 YdfR family protein -
JKV47_RS16250 (3364552) 3364552..3365085 + 534 WP_001092971.1 lysozyme -
JKV47_RS16255 (3365082) 3365082..3365579 + 498 WP_001071769.1 DUF2514 domain-containing protein -
JKV47_RS16260 (3365942) 3365942..3366154 + 213 WP_000066495.1 cold shock protein CspI -
JKV47_RS16265 (3366165) 3366165..3366353 + 189 WP_071524604.1 cold-shock protein -
JKV47_RS16270 (3366356) 3366356..3366421 + 66 WP_120795388.1 small protein YnfR -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3341291..3375235 33944


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T187508 WP_000323025.1 NZ_CP068680:3361162-3361449 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T187508 NZ_CP089877:3998249-3998467 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT187508 WP_000534858.1 NZ_CP068680:3360923-3361162 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT187508 NZ_CP089877:3997848-3998222 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9


Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References