Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 3107477..3109069 Replicon chromosome
Accession NZ_CP068678
Organism Pseudomonas aeruginosa strain NCCP15783

Toxin (Protein)


Gene name hipA Uniprot ID A0A550EV83
Locus tag JKV45_RS14490 Protein ID WP_034030705.1
Coordinates 3107477..3108802 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID W6RJ44
Locus tag JKV45_RS14495 Protein ID WP_003459718.1
Coordinates 3108803..3109069 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKV45_RS14450 (JKV45_14460) 3102828..3103070 + 243 WP_043884955.1 hypothetical protein -
JKV45_RS14455 (JKV45_14465) 3103334..3103699 + 366 WP_015503432.1 type II toxin-antitoxin system RelE/ParE family toxin -
JKV45_RS14460 (JKV45_14470) 3103692..3104015 + 324 WP_016561576.1 XRE family transcriptional regulator -
JKV45_RS14465 (JKV45_14475) 3104131..3104250 - 120 Protein_2851 IS5/IS1182 family transposase -
JKV45_RS14470 (JKV45_14480) 3104245..3104637 + 393 Protein_2852 hypothetical protein -
JKV45_RS14475 3104637..3105640 + 1004 Protein_2853 tyrosine-type recombinase/integrase -
JKV45_RS14480 (JKV45_14495) 3105857..3106108 + 252 WP_124136459.1 hypothetical protein -
JKV45_RS14485 (JKV45_14500) 3106138..3107004 + 867 WP_023081971.1 hypothetical protein -
JKV45_RS14490 (JKV45_14505) 3107477..3108802 + 1326 WP_034030705.1 HipA domain-containing protein Toxin
JKV45_RS14495 (JKV45_14510) 3108803..3109069 + 267 WP_003459718.1 helix-turn-helix transcriptional regulator Antitoxin
JKV45_RS14500 (JKV45_14515) 3109118..3109921 + 804 WP_079391650.1 inositol monophosphatase family protein -
JKV45_RS14505 (JKV45_14520) 3110212..3111129 - 918 WP_034031046.1 c-type cytochrome -
JKV45_RS14510 (JKV45_14525) 3111126..3111773 - 648 WP_034031048.1 c-type cytochrome -
JKV45_RS14515 (JKV45_14530) 3111831..3112607 - 777 WP_034031050.1 thiol:disulfide interchange protein DsbG -
JKV45_RS14520 (JKV45_14535) 3112604..3113086 - 483 Protein_2862 TlpA family protein disulfide reductase -
JKV45_RS14525 (JKV45_14540) 3113148..3113573 - 426 WP_017940147.1 YeeE/YedE family protein -
JKV45_RS14530 (JKV45_14545) 3113583..3114014 - 432 WP_017940146.1 YeeE/YedE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3107477..3144856 37379


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-385)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 49225.86 Da        Isoelectric Point: 5.3507

>T187483 WP_034030705.1 NZ_CP068678:3107477-3108802 [Pseudomonas aeruginosa]
MHNLTLQVYRDGQWRNAMELRFEQPEEGLEGACAYGYQQGYLVDSLESVGTRLNAAVSAAYPLGWDSWRDKKAPAFLFDI
LPSGAARRFLLKRLGGERPEGLNLDLYLLGRCTPAPIGNLRIKESVEAIAGSPVLGFTRDEVISRDSRFLEYAYEQGAAI
GGATGAGGEAPKLLLTEDRHGALHPDAVLPDADAVQHWFVKFARNKASQTDQDILRSEYCFYRAVQQLGMDTVAAEGLAL
EEGNKPSLWMHRFDREISSTGVCRTAVESMYSLCGVTEAGSYMLHTQVLGKLVELWRAVGQEQQVGDLVFEYMRRDLLNQ
ILGNSDNHGRNTAILRSEQGVRLAPIYDLAPMVMDEEGVTRTTKWPKHIELAGEVNWKRACDEVAGWVNADELYQRLRLA
AQDFLALPDLLSAQGLPDSTMSHPRIALRNLRQRMLQWGLV

Download         Length: 1326 bp

>T187483 NZ_CP089870:c4720695-4720411 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9933.55 Da        Isoelectric Point: 10.9502

>AT187483 WP_003459718.1 NZ_CP068678:3108803-3109069 [Pseudomonas aeruginosa]
MDILERNRILNELQQQLASGEITIGGAVRRLRKEVTGLQQARFAQMCKLSLRALRQLELDESNPTVQTLNSVFNPFGMQV
GIVPKTPR

Download         Length: 267 bp

>AT187483 NZ_CP089870:c4720927-4720685 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A550EV83

Antitoxin

Source ID Structure
AlphaFold DB W6RJ44

References