Detailed information of TA system TA187481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 3003957..3004565
• Replicon: chromosome
• Accession: NZ_CP068678
• Organism: Pseudomonas aeruginosa strain NCCP15783

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: JKV45_RS13975
• Protein ID: WP_016263850.1
• Coordinates: 3004218..3004565 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: JKV45_RS13970
• Protein ID: WP_003114155.1
• Coordinates: 3003957..3004208 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKV45_RS13950 (JKV45_13960)	2999595..2999945	+	351	WP_019725830.1	DUF2523 family protein	-
JKV45_RS13955 (JKV45_13965)	2999947..3001209	+	1263	WP_019725831.1	zonular occludens toxin domain-containing protein	-
JKV45_RS13960 (JKV45_13970)	3001468..3002760	+	1293	WP_003115206.1	hypothetical protein	-
JKV45_RS13965 (JKV45_13975)	3002760..3003743	+	984	WP_019725833.1	tyrosine-type recombinase/integrase	-
JKV45_RS13970 (JKV45_13980)	3003957..3004208	+	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JKV45_RS13975 (JKV45_13985)	3004218..3004565	+	348	WP_016263850.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JKV45_RS13985 (JKV45_13995)	3004892..3005794	-	903	WP_003123042.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
JKV45_RS13990 (JKV45_14000)	3006246..3006983	-	738	WP_003114158.1	murein L,D-transpeptidase catalytic domain family protein	-
JKV45_RS13995 (JKV45_14005)	3007063..3008106	-	1044	WP_003098363.1	L,D-transpeptidase	-
JKV45_RS14000 (JKV45_14010)	3008242..3008934	-	693	WP_003098362.1	16S rRNA pseudouridine(516) synthase	-
JKV45_RS14005 (JKV45_14015)	3008934..3009206	-	273	WP_019725834.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2990031..3004565	14534

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13046.85 Da
• Isoelectric Point: 4.4212

>T187481 WP_016263850.1 NZ_CP068678:3004218-3004565 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLVPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T187481 NZ_CP089870:3980717-3980935 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT187481 WP_003114155.1 NZ_CP068678:3003957-3004208 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT187481 NZ_CP089870:3980316-3980690 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTAGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355