Detailed information of TA system TA187480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1599594..1600099
• Replicon: chromosome
• Accession: NZ_CP068678
• Organism: Pseudomonas aeruginosa strain NCCP15783

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: JKV45_RS07430
• Protein ID: WP_003083773.1
• Coordinates: 1599594..1599875 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: JKV45_RS07435
• Protein ID: WP_003083775.1
• Coordinates: 1599872..1600099 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKV45_RS07405 (JKV45_07400)	1594845..1596194	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
JKV45_RS07410 (JKV45_07405)	1596243..1596929	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
JKV45_RS07415 (JKV45_07410)	1597030..1597764	+	735	WP_003117208.1	GntR family transcriptional regulator	-
JKV45_RS07420 (JKV45_07415)	1597944..1598354	+	411	WP_003101225.1	aegerolysin family protein	-
JKV45_RS07425 (JKV45_07420)	1598386..1599294	-	909	WP_003083769.1	LysR family transcriptional regulator	-
JKV45_RS07430 (JKV45_07425)	1599594..1599875	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
JKV45_RS07435 (JKV45_07430)	1599872..1600099	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
JKV45_RS07440 (JKV45_07435)	1600275..1600895	-	621	WP_009314940.1	hypothetical protein	-
JKV45_RS07445 (JKV45_07440)	1600996..1601496	+	501	WP_003112629.1	LEA type 2 family protein	-
JKV45_RS07450 (JKV45_07445)	1601569..1601910	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
JKV45_RS07455 (JKV45_07450)	1601992..1603419	-	1428	WP_003083784.1	GABA permease	-
JKV45_RS07460 (JKV45_07455)	1603588..1605081	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T187480 WP_003083773.1 NZ_CP068678:c1599875-1599594 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T187480 NZ_CP068678:c1599875-1599594 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT187480 WP_003083775.1 NZ_CP068678:c1600099-1599872 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT187480 NZ_CP068678:c1600099-1599872 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9