Detailed information of TA system TA187478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 22498..23014
• Replicon: plasmid pTJ187-3
• Accession: NZ_CP068651
• Organism: Vibrio parahaemolyticus strain TJ-187

Toxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JHS88_RS25250
• Protein ID: WP_140220082.1
• Coordinates: 22745..23014 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: U2ZH00
• Locus tag: JHS88_RS25245
• Protein ID: WP_005526636.1
• Coordinates: 22498..22752 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHS88_RS25230 (JHS88_25205)	17940..18560	+	621	WP_140391890.1	recombinase family protein	-
JHS88_RS25235 (JHS88_25210)	18564..21560	+	2997	WP_140391851.1	Tn3 family transposase	-
JHS88_RS25240 (JHS88_25215)	21590..22165	-	576	Protein_22	recombinase family protein	-
JHS88_RS25245 (JHS88_25220)	22498..22752	+	255	WP_005526636.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
JHS88_RS25250 (JHS88_25225)	22745..23014	+	270	WP_140220082.1	Txe/YoeB family addiction module toxin	Toxin
JHS88_RS25255 (JHS88_25230)	23532..23801	+	270	WP_140220080.1	transposase	-
JHS88_RS25260 (JHS88_25235)	23831..24514	-	684	WP_140220078.1	IS6 family transposase	-
JHS88_RS25265 (JHS88_25240)	24584..25402	+	819	Protein_27	IS3 family transposase	-
JHS88_RS25270 (JHS88_25245)	25565..25999	-	435	WP_140220074.1	hypothetical protein	-
JHS88_RS25275 (JHS88_25250)	26079..26291	-	213	WP_140220072.1	hypothetical protein	-
JHS88_RS25280 (JHS88_25255)	26447..26647	+	201	Protein_30	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	trhX	1..86558	86558

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (10-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10618.95 Da
• Isoelectric Point: 7.9816

>T187478 WP_140220082.1 NZ_CP068651:22745-23014 [Vibrio parahaemolyticus]
MSSSQRLLSWTDDAWDDYLYWQTQDKKTLKRINKLINDIKRSSFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDQAI
TIISCRYHY

Nucleotide

• Length: 270 bp

>T187478 NZ_CP089870:c1844867-1844451 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGGGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCACCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTACCGGGCCTGGTGCTGG
AAGACTGGGTAAAATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9453.60 Da
• Isoelectric Point: 5.0623

>AT187478 WP_005526636.1 NZ_CP068651:22498-22752 [Vibrio parahaemolyticus]
MRIVSFTEARNGLKAVLDGVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPQNAEHLNRSIAQYRAGKTTARE
LIDE

Nucleotide

• Length: 255 bp

>AT187478 NZ_CP089870:c1845094-1844864 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGTGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A244CM06