Detailed information of TA system TA187477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 51622..52138
• Replicon: plasmid pTJ187-2
• Accession: NZ_CP068650
• Organism: Vibrio parahaemolyticus strain TJ-187

Toxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JHS88_RS25010
• Protein ID: WP_140220082.1
• Coordinates: 51622..51891 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: U2ZH00
• Locus tag: JHS88_RS25015
• Protein ID: WP_005526636.1
• Coordinates: 51884..52138 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHS88_RS24980 (JHS88_24955)	47989..48189	-	201	Protein_48	recombinase family protein	-
JHS88_RS24985 (JHS88_24960)	48345..48557	+	213	WP_140220072.1	hypothetical protein	-
JHS88_RS24990 (JHS88_24965)	48637..49071	+	435	WP_140220074.1	hypothetical protein	-
JHS88_RS24995 (JHS88_24970)	49234..50052	-	819	Protein_51	IS3 family transposase	-
JHS88_RS25000 (JHS88_24975)	50122..50805	+	684	WP_140220078.1	IS6 family transposase	-
JHS88_RS25005 (JHS88_24980)	50835..51104	-	270	WP_140220080.1	transposase	-
JHS88_RS25010 (JHS88_24985)	51622..51891	-	270	WP_140220082.1	Txe/YoeB family addiction module toxin	Toxin
JHS88_RS25015 (JHS88_24990)	51884..52138	-	255	WP_005526636.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
JHS88_RS25020 (JHS88_24995)	52471..53046	+	576	Protein_56	recombinase family protein	-
JHS88_RS25025 (JHS88_25000)	53076..56072	-	2997	WP_140391851.1	Tn3 family transposase	-
JHS88_RS25030 (JHS88_25005)	56076..56696	-	621	WP_140391890.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71764	71764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (10-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10618.95 Da
• Isoelectric Point: 7.9816

>T187477 WP_140220082.1 NZ_CP068650:c51891-51622 [Vibrio parahaemolyticus]
MSSSQRLLSWTDDAWDDYLYWQTQDKKTLKRINKLINDIKRSSFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDQAI
TIISCRYHY

Nucleotide

• Length: 270 bp

>T187477 NZ_CP089870:802338-802748 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9453.60 Da
• Isoelectric Point: 5.0623

>AT187477 WP_005526636.1 NZ_CP068650:c52138-51884 [Vibrio parahaemolyticus]
MRIVSFTEARNGLKAVLDGVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPQNAEHLNRSIAQYRAGKTTARE
LIDE

Nucleotide

• Length: 255 bp

>AT187477 NZ_CP089870:802091-802357 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A244CM06