Detailed information of TA system TA187466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2794961..2795724
• Replicon: chromosome
• Accession: NZ_CP068633
• Organism: Vibrio parahaemolyticus strain VP17

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A919BFZ0
• Locus tag: JHT19_RS13330
• Protein ID: WP_023584043.1
• Coordinates: 2795218..2795724 (+)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: JHT19_RS13325
• Protein ID: WP_000212004.1
• Coordinates: 2794961..2795227 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHT19_RS13305 (JHT19_13265)	2790665..2791177	-	513	WP_205395245.1	S26 family signal peptidase	-
JHT19_RS13310 (JHT19_13270)	2791161..2791508	-	348	WP_025516117.1	hypothetical protein	-
JHT19_RS13315 (JHT19_13275)	2791501..2793900	-	2400	WP_028867951.1	type IV secretion system protein TraC	-
JHT19_RS13320 (JHT19_13280)	2793900..2794592	-	693	WP_114867802.1	DsbC family protein	-
JHT19_RS13325 (JHT19_13285)	2794961..2795227	+	267	WP_000212004.1	DUF1778 domain-containing protein	Antitoxin
JHT19_RS13330 (JHT19_13290)	2795218..2795724	+	507	WP_023584043.1	GNAT family N-acetyltransferase	Toxin
JHT19_RS13335 (JHT19_13295)	2795765..2796151	-	387	WP_023584042.1	TraA family conjugative transfer protein	-
JHT19_RS13340 (JHT19_13300)	2796148..2796720	-	573	WP_146008853.1	type IV conjugative transfer system lipoprotein TraV	-
JHT19_RS13345 (JHT19_13305)	2796795..2798084	-	1290	WP_114867803.1	TraB/VirB10 family protein	-
JHT19_RS13350 (JHT19_13310)	2798087..2798983	-	897	WP_114867987.1	type-F conjugative transfer system secretin TraK	-
JHT19_RS13355 (JHT19_13315)	2798967..2799593	-	627	WP_114867804.1	TraE/TraK family type IV conjugative transfer system protein	-
JHT19_RS13360 (JHT19_13320)	2799590..2799871	-	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
JHT19_RS13365 (JHT19_13325)	2799996..2800442	+	447	WP_114867805.1	hypothetical protein	-
JHT19_RS13370 (JHT19_13330)	2800446..2800709	+	264	WP_114867806.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-83)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 18278.00 Da
• Isoelectric Point: 7.2132

>T187466 WP_023584043.1 NZ_CP068633:2795218-2795724 [Vibrio parahaemolyticus]
MGISAPTLLTDEHQLNAFQCGIEELDTWLRKQALKSQKRGTARVYVVNDTDAHQVVGYYAIAMGSVSREQAFSSLRRNSP
DPIPMVILARLGVDNAYQGQGIAAGLLKDCIIRSVQAMNAVGGAGILVHAIDGSAQTFYKKFGFKESTFDPLVLMARICD
IEKSLSID

Nucleotide

• Length: 507 bp

>T187466 NZ_CP089849:1174840-1175130 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9956.48 Da
• Isoelectric Point: 5.8580

>AT187466 WP_000212004.1 NZ_CP068633:2794961-2795227 [Vibrio parahaemolyticus]
MATKTAPINMRVLPSVRDIIDAAASLKKVDRTVFIQQAALNEAHSILAEQRDFVLEAKAFEAFDNELRAEPQTLEGMKDL
FKRKAPWE

Nucleotide

• Length: 267 bp

>AT187466 NZ_CP089849:1174544-1174843 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA