Detailed information of TA system TA187447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 21068..21711
• Replicon: plasmid p41968-R65.8
• Accession: NZ_CP068621
• Organism: Klebsiella pneumoniae strain 41968

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: JMZ79_RS27620
• Protein ID: WP_001044770.1
• Coordinates: 21068..21484 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: JMZ79_RS27625
• Protein ID: WP_001261282.1
• Coordinates: 21481..21711 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMZ79_RS27585	16610..16846	-	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-
JMZ79_RS27590	16843..17205	-	363	WP_001277456.1	mercury resistance co-regulator MerD	-
JMZ79_RS27595	17223..18917	-	1695	WP_000105636.1	mercury(II) reductase	-
JMZ79_RS27600	18969..19391	-	423	WP_001340589.1	organomercurial transporter MerC	-
JMZ79_RS27605	19427..19537	-	111	Protein_22	mercuric transport protein periplasmic component	-
JMZ79_RS27610	19553..20250	-	698	Protein_23	IS1 family transposase	-
JMZ79_RS27615	20284..20994	-	711	WP_004152334.1	ATP-binding protein	-
JMZ79_RS27620	21068..21484	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
JMZ79_RS27625	21481..21711	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JMZ79_RS27630	21668..22129	+	462	WP_072202616.1	hypothetical protein	-
JMZ79_RS27635	22273..22623	+	351	WP_000493378.1	hypothetical protein	-
JMZ79_RS27640	22674..23417	+	744	WP_000129823.1	hypothetical protein	-
JMZ79_RS27645	23414..24190	+	777	WP_000015958.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-3 / blaOXA-9 / blaTEM-1A / aac(6')-Ib / aac(3)-IVa / aph(4)-Ia	-	1..65819	65819
	-	inside	IScluster/Tn	aac(6')-Ib	-	19553..30029	10476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T187447 WP_001044770.1 NZ_CP068621:c21484-21068 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T187447 NZ_CP089799:195438-195923 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGGGACATGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCAACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGAAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT187447 WP_001261282.1 NZ_CP068621:c21711-21481 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT187447 NZ_CP089799:195163-195450 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACATGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3